Melo Luiz G N, Nagata Maria J H, Bosco Alvaro F, Ribeiro Luciana L G, Leite Cristiane M
Division of Periodontics, Department of Surgery and Integrated Clinic, Dental School of Araçatuba, University of State of São Paulo Júlio de Mesquita Filho-UNESP, Araçatuba, Brazil.
Clin Oral Implants Res. 2005 Dec;16(6):683-91. doi: 10.1111/j.1600-0501.2005.01090.x.
The purpose of this study was to histologically analyze the influence of bioactive glass and/or a calcium sulfate barrier on bone healing in surgically created defects in rat tibias.
Sixty-four rats were divided into 4 groups: C (control), CS (calcium sulfate), BG (bioactive glass), and BG/CS (bioactive glass/calcium sulfate). A surgical defect was created in the tibia of each animal. In Group CS, a calcium sulfate barrier was placed to cover the defect. In Group BG the defect was filled with bioactive glass. In Group BG/CS, it was filled with bioactive glass and protected by a barrier of calcium sulfate. Animals were sacrificed at 10 or 30 days post-operative. The formation of new bone in the cortical area of the defect was evaluated histomorphometrically.
At 10 days post-operative, Group C presented significantly more bone formation than Groups CS, BG, or BG/CS. No statistically significant differences were found between the experimental groups. At 30 days post-operative, Group C demonstrated significantly more bone formation than the experimental groups. Groups CS and BG/CS showed significantly more bone formation than Group BG. No statistically significant differences were found between Group CS and BG/CS.
(a) the control groups had significantly more bone formation than the experimental groups; (b) at 10 days post-operative, no significant differences were found between any of the experimental groups; and (c) at 30 days post-operative, the groups with a calcium sulfate barrier had significantly more bone formation than the group that used bioactive glass only.
本研究旨在通过组织学分析生物活性玻璃和/或硫酸钙屏障对大鼠胫骨手术造成的缺损处骨愈合的影响。
64只大鼠分为4组:C组(对照组)、CS组(硫酸钙组)、BG组(生物活性玻璃组)和BG/CS组(生物活性玻璃/硫酸钙组)。对每只动物的胫骨制造一个手术缺损。在CS组中,放置硫酸钙屏障覆盖缺损处。在BG组中,缺损处填充生物活性玻璃。在BG/CS组中,缺损处填充生物活性玻璃并用硫酸钙屏障保护。术后10天或30天处死动物。对缺损处皮质区域新骨形成进行组织形态计量学评估。
术后10天,C组的骨形成明显多于CS组、BG组或BG/CS组。各实验组之间未发现统计学上的显著差异。术后30天,C组的骨形成明显多于各实验组。CS组和BG/CS组的骨形成明显多于BG组。CS组和BG/CS组之间未发现统计学上的显著差异。
(a)对照组的骨形成明显多于实验组;(b)术后10天,各实验组之间未发现显著差异;(c)术后30天,有硫酸钙屏障的组的骨形成明显多于仅使用生物活性玻璃的组。