Hur Gyu-Young, Choi Gil-Soon, Sheen Seung-Soo, Lee Hyun-Young, Park Han-Jung, Choi Sung-Jin, Ye Young-Min, Park Hae-Sim
Department of Allergy and Rheumatology, Ajou University School of Medicine, Suwon, South Korea.
Pulmonary and Critical Care Medicine, Ajou University School of Medicine, Suwon, South Korea.
J Allergy Clin Immunol. 2008 Oct;122(4):774-780. doi: 10.1016/j.jaci.2008.07.034.
Although methylene diphenyl diisocyanate (MDI) may induce occupational asthma in the workplace, the pathogenic mechanisms are unclear.
By using bronchoalveolar lavage fluid, we sought to identify proteins that were differentially expressed between subjects with MDI-induced occupational asthma (MDI-OA) and asymptomatic exposed controls (AECs).
To find proteins that were differentially expressed between the MDI-OA and AEC groups, 2-dimensional electrophoresis was performed by using bronchoalveolar lavage fluid obtained from subjects after MDI-specific inhalation challenge. The selected protein spots were then identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The clinical relevance of the differentially expressed spots was compared by ELISA using sera from the MDI-OA/eosinophilic bronchitis, AEC, and unexposed healthy control groups. Receiver operating characteristic curves were then plotted, and the sensitivity and specificity were determined.
Twenty-three protein spots were identified that distinguished the subjects with MDI-OA from those in the AEC group. Among them, ferritin expression was downregulated whereas transferrin expression was upregulated in subjects with MDI-OA compared with AEC; these results were validated by ELISA using sera from the MDI-OA/EB and AEC groups. To identify subjects with MDI-OA, the optimal serum cutoff levels were 69.84 ng/mL for ferritin and 2.48 microg/mL for transferrin. When these 2 parameters were combined, the sensitivity was 71.43% and the specificity was 85.71%.
Serum ferritin and transferrin levels are associated with the phenotype of MDI-OA.
尽管二苯基甲烷二异氰酸酯（MDI）可能在工作场所诱发职业性哮喘，但其致病机制尚不清楚。
通过支气管肺泡灌洗，我们试图鉴定在MDI诱发的职业性哮喘（MDI-OA）患者和无症状暴露对照者（AEC）之间差异表达的蛋白质。
为了找出MDI-OA组和AEC组之间差异表达的蛋白质，对MDI特异性吸入激发后受试者的支气管肺泡灌洗进行二维电泳。然后通过基质辅助激光解吸/电离飞行时间质谱法鉴定所选蛋白质点。使用来自MDI-OA/嗜酸性支气管炎、AEC和未暴露健康对照组的血清，通过ELISA比较差异表达点的临床相关性。然后绘制受试者工作特征曲线，并确定敏感性和特异性。
鉴定出23个蛋白质点，可区分MDI-OA患者和AEC组患者。其中，与AEC相比，MDI-OA患者的铁蛋白表达下调，而转铁蛋白表达上调；使用来自MDI-OA/EB和AEC组的血清通过ELISA验证了这些结果。为了鉴定MDI-OA患者，铁蛋白的最佳血清截断水平为69.84 ng/mL，转铁蛋白为2.48 μg/mL。当这两个参数结合时，敏感性为71.43%，特异性为85.71%。
血清铁蛋白和转铁蛋白水平与MDI-OA的表型有关。
