College of Pharmacy, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
College of Life Sciences, Liaoning University, Shenyang, Liaoning 110000, P.R. China.
Int J Mol Med. 2018 Sep;42(3):1273-1282. doi: 10.3892/ijmm.2018.3717. Epub 2018 Jun 5.
The aim of the present study was to prepare spirulina polysaccharide (PSP) into an oral nanoemulsion (NE) with the aim of improving its oral bioavailability and prolonging its sustained release effect. The PSP‑NE was prepared through a phase transformation method, and its formulation components were screened through the use of a pseudo‑ternary phase diagram. The optimal formulation of PSP‑NE was determined to be: 11.9% Span 80, 6.0% Tween-80, 9.0% ethanol, 62.8% soybean oil, and 10.3% PSP aqueous solution. The prepared PSP‑NE was clear and transparent, had a uniform color and spherical morphology, exhibited stability and no adhesion. The average particle size was 79.93±19 nm, the polydispersity index was 0.185±0.04 (n=3), and the entrapment rate was 62%. Small‑animal imaging results showed that the prepared PSP‑NE exhibited a sustained release and tissue effect in contrast to the PSP aqueous solution. The present study showed that the prepared PSP‑NE not only exhibited a sustained release and tissue effect in contrast to the PSP aqueous solution, but also had superior performance in terms of antitumor and antioxidant effects.
本研究旨在将螺旋藻多糖（PSP）制备成口服纳米乳（NE），以提高其口服生物利用度并延长其持续释放效果。通过相转变法制备 PSP-NE，并通过伪三元相图筛选其配方成分。确定 PSP-NE 的最佳配方为：11.9%Span80、6.0%Tween-80、9.0%乙醇、62.8%大豆油和 10.3%PSP 水溶液。所制备的 PSP-NE 澄清透明，颜色均匀，呈球形形态，表现出稳定性且无粘连。平均粒径为 79.93±19nm，多分散指数为 0.185±0.04（n=3），包封率为 62%。小动物成像结果表明，与 PSP 水溶液相比，所制备的 PSP-NE 表现出持续释放和组织效应。本研究表明，与 PSP 水溶液相比，所制备的 PSP-NE 不仅表现出持续释放和组织效应，而且在抗肿瘤和抗氧化作用方面具有更好的性能。
