Bai Zhijun, Liu Licheng, Tu Zeng, Yao Lisi, Liu Jianwei, Xu Bing, Tang Boheng, Liu Jinhua, Wan Yongji, Fang Meiyu, Chen Weijun
Guangzhou Medical Research Institute, Yan-Ling, Guangzhou 510507, PR China.
Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, PR China.
J Med Microbiol. 2008 Dec;57(Pt 12):1547-1552. doi: 10.1099/jmm.0.2008/003418-0.
Dengue virus (DENV) causes a wide range of diseases in humans, from the acute febrile illness dengue fever (DF) to life-threatening dengue haemorrhagic fever/dengue shock syndrome. We developed four real-time quantitative PCR assays for each serotype of DENV based on computational analysis. These assays had high sensitivity and specificity without cross-reactivity for the four serotypes. To evaluate the performance of these assays in detecting and typing the virus in clinical samples, we analysed 64 serum samples from Guangdong during 2006. The results showed that 71 % of those samples were positive by the DEN-1 assay. The DENV assay results, in agreement with the serological tests and sequencing analysis, showed that the pathogen resulting in the DF explosion in Guangdong in 2006 belonged to DEN-1. Compared to the serological assays, the real-time PCR assays that we developed were much more sensitive in the 1-3 days after onset of the symptoms.
登革病毒（DENV）可导致人类多种疾病，从急性发热性疾病登革热（DF）到危及生命的登革出血热/登革休克综合征。我们基于计算分析为DENV的每种血清型开发了四种实时定量PCR检测方法。这些检测方法具有高灵敏度和特异性，对四种血清型无交叉反应。为了评估这些检测方法在临床样本中检测和分型病毒的性能，我们分析了2006年期间来自广东的64份血清样本。结果显示，71%的样本通过DEN-1检测呈阳性。DENV检测结果与血清学检测和测序分析一致，表明2006年在广东导致登革热爆发的病原体属于DEN-1。与血清学检测相比，我们开发的实时PCR检测方法在症状出现后的1-3天内灵敏度更高。
