Soltis S Michael, Cohen Aina E, Deacon Ashley, Eriksson Thomas, González Ana, McPhillips Scott, Chui Hsui, Dunten Pete, Hollenbeck Michael, Mathews Irimpan, Miller Mitch, Moorhead Penjit, Phizackerley R Paul, Smith Clyde, Song Jinhu, van dem Bedem Henry, Ellis Paul, Kuhn Peter, McPhillips Timothy, Sauter Nicholas, Sharp Kenneth, Tsyba Irina, Wolf Guenter
SSRL, SLAC, 2575 Sand Hill Road MS 99, Menlo Park, CA 95124, USA.
Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1210-21. doi: 10.1107/S0907444908030564. Epub 2008 Nov 18.
Complete automation of the macromolecular crystallography experiment has been achieved at SSRL through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface. These highly reliable systems have enabled crystallography experiments to be carried out from the researchers' home institutions and other remote locations while retaining complete control over even the most challenging systems. A breakthrough component of the system, the Stanford Auto-Mounter (SAM), has enabled the efficient mounting of cryocooled samples without human intervention. Taking advantage of this automation, researchers have successfully screened more than 200 000 samples to select the crystals with the best diffraction quality for data collection as well as to determine optimal crystallization and cryocooling conditions. These systems, which have been deployed on all SSRL macromolecular crystallography beamlines and several beamlines worldwide, are used by more than 80 research groups in remote locations, establishing a new paradigm for macromolecular crystallography experimentation.
通过将坚固的机械化实验硬件与具有直观用户界面的灵活控制系统相结合，SSRL已实现了大分子晶体学实验的完全自动化。这些高度可靠的系统使晶体学实验能够在研究人员的所属机构及其他远程地点进行，同时即使是对最具挑战性的系统也能保持完全控制。该系统的一个突破性组件——斯坦福自动挂载仪（SAM），能够在无需人工干预的情况下高效挂载低温冷却样品。利用这种自动化技术，研究人员已成功筛选了超过20万个样品，以选择具有最佳衍射质量的晶体进行数据收集，并确定最佳的结晶和低温冷却条件。这些系统已部署在SSRL所有的大分子晶体学光束线以及全球多条光束线上，被80多个位于远程地点的研究团队使用，开创了大分子晶体学实验的新模式。
