儿童急性非复杂性临床疟疾感染后外周血B细胞亚群的变化
Alterations on peripheral B cell subsets following an acute uncomplicated clinical malaria infection in children.
作者信息
Asito Amolo S, Moormann Ann M, Kiprotich Chelimo, Ng'ang'a Zipporah W, Ploutz-Snyder Robert, Rochford Rosemary
机构信息
School of Pure and Applied Science, Kenyatta University, Nairobi, Kenya.
出版信息
Malar J. 2008 Nov 18;7:238. doi: 10.1186/1475-2875-7-238.
BACKGROUND
The effects of Plasmodium falciparum on B-cell homeostasis have not been well characterized. This study investigated whether an episode of acute malaria in young children results in changes in the peripheral B cell phenotype.
METHODS
Using flow-cytofluorimetric analysis, the B cell phenotypes found in the peripheral blood of children aged 2-5 years were characterized during an episode of acute uncomplicated clinical malaria and four weeks post-recovery and in healthy age-matched controls.
RESULTS
There was a significant decrease in CD19+ B lymphocytes during acute malaria. Characterization of the CD19+ B cell subsets in the peripheral blood based on expression of IgD and CD38 revealed a significant decrease in the numbers of naive 1 CD38-IgD+ B cells while there was an increase in CD38+IgD- memory 3 B cells during acute malaria. Further analysis of the peripheral B cell phenotype also identified an expansion of transitional CD10+CD19+ B cells in children following an episode of acute malaria with up to 25% of total CD19+ B cell pool residing in this subset.
CONCLUSION
Children experiencing an episode of acute uncomplicated clinical malaria experienced profound disturbances in B cell homeostasis.
背景
恶性疟原虫对B细胞稳态的影响尚未得到充分表征。本研究调查了幼儿急性疟疾发作是否会导致外周B细胞表型的变化。
方法
采用流式细胞荧光分析，对2至5岁儿童在急性非复杂性临床疟疾发作期间、康复后四周以及年龄匹配的健康对照者外周血中发现的B细胞表型进行了表征。
结果
急性疟疾期间，CD19+B淋巴细胞显著减少。根据IgD和CD38的表达对外周血中CD19+B细胞亚群进行表征，结果显示，急性疟疾期间，幼稚1型CD38-IgD+B细胞数量显著减少，而CD38+IgD-记忆3型B细胞数量增加。对外周B细胞表型的进一步分析还发现，急性疟疾发作后的儿童过渡性CD10+CD19+B细胞扩增，该亚群占总CD19+B细胞池的比例高达25%。
结论
经历急性非复杂性临床疟疾发作的儿童B细胞稳态受到严重干扰。
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