Aboudharam Gérard, Vu Dang La, Davoust Bernard, Drancourt Michel, Raoult Didier
Unité des Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, 27, Boulevard Jean Moulin, Marseille Cedex 05 13385, France.
Microb Pathog. 2005 Jan;38(1):47-51. doi: 10.1016/j.micpath.2004.10.004. Epub 2004 Dec 8.
Bartonella henselae causes chronic bacteremia in cats. To test if B. henselae DNA can be recovered from the dental pulp of cats buried a year previously, we used PCR with primers for a sequence of the conserved groEL to test 104 teeth from 11 cats. Seven of the cats were found positive; canine teeth were more frequently positive than molar teeth. Where PCR sequences could be determined, they were identical to those of B. henselae Marseille (four cats), B. henselae Houston (one cat) or similar to those of B. grahamii (one cat). Our study indicates that dental pulp from the teeth of cats, especially the canine teeth, may be used for the PCR detection of Bartonella in animals buried for a year.
汉赛巴尔通体可导致猫慢性菌血症。为检测能否从一年前埋葬的猫的牙髓中提取到汉赛巴尔通体DNA，我们使用针对保守的groEL序列的引物进行PCR，检测了11只猫的104颗牙齿。发现其中7只猫呈阳性；犬齿比臼齿更频繁地呈阳性。在能够确定PCR序列的情况下，它们与汉赛巴尔通体马赛株（4只猫）、汉赛巴尔通体休斯顿株（1只猫）的序列相同，或与格雷厄姆巴尔通体的序列相似（1只猫）。我们的研究表明，猫牙齿的牙髓，尤其是犬齿，可用于对埋葬一年的动物进行巴尔通体的PCR检测。
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