Lei Ming, Yao Baoli
State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China.
J Biomed Opt. 2008 Jul-Aug;13(4):044024. doi: 10.1117/1.2960019.
We present a multifunctional darkfield microscopy using an axicon. It combines the functions of a darkfield microscope, fluorescence microscope, and microspectrophotometer in one platform. The advantage of the system over conventional darkfield microscopy includes the high transmittance of the illuminating flux, the high contrast of the image, and the convenient toggle between darkfield and brightfield microscopy. Examples of dark, bright, and fluorescent micrographs as well as concerned spectra of microsized specimens implemented in this apparatus are demonstrated.
我们展示了一种使用轴锥镜的多功能暗场显微镜。它在一个平台上结合了暗场显微镜、荧光显微镜和显微分光光度计的功能。该系统相对于传统暗场显微镜的优势包括照明通量的高透射率、图像的高对比度以及暗场和明场显微镜之间方便的切换。展示了在此仪器中实现的微尺寸标本的暗场、明场和荧光显微照片示例以及相关光谱。
