Moustafa Tarek E, Polanco Edward R, Belote Rachel L, Judson-Torres Robert L, Zangle Thomas A
Department of Chemical Engineering, University of Utah, Salt Lake City, UT, USA.
Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA.
HardwareX. 2023 Jan 24;13:e00399. doi: 10.1016/j.ohx.2023.e00399. eCollection 2023 Mar.
The combination of multiple imaging modalities in a single microscopy system can enable new insights into biological processes. In this work, we describe the construction and rigorous characterization of a custom microscope with multimodal imaging in a single, cost-effective system. Our design utilizes advances in LED technology, robotics, and open-source software, along with existing optical components and precision optomechanical parts to offer a modular and versatile design. This microscope is operated using software written in Arduino and Python and has the ability to run multi-day automated imaging experiments when placed inside of a cell culture incubator. Additionally, we provide and demonstrate methods to validate images taken in brightfield and darkfield, along with validation and optimization for differential phase contrast (DPC) quantitative phase imaging.
在单个显微镜系统中结合多种成像方式能够为生物过程带来新的见解。在这项工作中，我们描述了一个定制显微镜的构建及严格表征，该显微镜在一个经济高效的单一系统中具备多模态成像功能。我们的设计利用了LED技术、机器人技术和开源软件方面的进展，以及现有的光学元件和精密光机械部件，以提供模块化且通用的设计。此显微镜使用用Arduino和Python编写的软件进行操作，当放置在细胞培养箱内时，有能力运行多天的自动成像实验。此外，我们提供并展示了验证明场和暗场拍摄图像的方法，以及差分相衬（DPC）定量相成像的验证和优化方法。
