Sun Jian, Chan Chi-Chiu, Zhang Yi-Fan, Shum Ping
Nanyang Technological University, School of Chemical and Biomedical Engineering, Division of Bioengineering, Singapore, 637457.
J Biomed Opt. 2008 Sep-Oct;13(5):054048. doi: 10.1117/1.2983676.
Hollow-core photonic bandgap fiber (HC-PBGF)-based evanescent wave biosensors are demonstrated and analyzed theoretically and experimentally. With 95% of the guided light power residing in the samples, the measured absorbance for a 30-cm-long fiber filled with a 0.2 microM Alexa Fluor 700-labeled DNA Oligo solution is 1.06. This is in good agreement with the theoretical prediction, which is evaluated by using the refractive index scaling law. The HC-PBGFs thus offer both efficiency and simplicity for the detection of biomolecules in ultra-small sample volumes.
本文展示、理论分析并实验研究了基于空芯光子带隙光纤（HC-PBGF）的倏逝波生物传感器。对于一根填充了0.2微摩尔用 Alexa Fluor 700标记的DNA寡核苷酸溶液的30厘米长光纤，95%的导光功率存在于样品中，测得的吸光度为1.06。这与使用折射率比例定律评估得到的理论预测结果高度吻合。因此，空芯光子带隙光纤在超小样品体积生物分子检测方面兼具高效性与简易性。
