McAllister Ryan G, Sisan Daniel R, Urbach Jeffrey S
Georgetown University, Department of Physics, Washington, DC 20057, USA.
J Biomed Opt. 2008 Sep-Oct;13(5):054058. doi: 10.1117/1.2992141.
We describe the principles, design, and systems integration of a flexible, high-speed, high-sensitivity, high-resolution confocal spinning disk microscopy (SDCM) system. We present several artifacts unique to high-speed SDCM along with techniques to minimize them. We show example experimental results from a specific implementation capable of generating 3-D image stacks containing 30 2-D slices at 30 stacks per second. This implementation also includes optics for differential interference contrast (DIC), phase, and bright-field imaging, as well as an optical trap with sensitive force and position measurement.
我们描述了一种灵活、高速、高灵敏度、高分辨率的共聚焦旋转盘显微镜（SDCM）系统的原理、设计和系统集成。我们介绍了高速SDCM特有的几种伪像以及将其最小化的技术。我们展示了一个特定实现的示例实验结果，该实现能够以每秒30帧的速度生成包含30个二维切片的三维图像堆栈。此实现还包括用于微分干涉对比（DIC）、相位和明场成像的光学器件，以及具有灵敏力和位置测量功能的光阱。
