Gräf Ralph, Rietdorf Jens, Zimmermann Timo
A.-Butenandt-Institut/Zellbiologie, Ludwig-Maximilians-Universität München, Schillerstrasse 42,80336 München, Germany.
Adv Biochem Eng Biotechnol. 2005;95:57-75. doi: 10.1007/b102210.
In vivo microscopy of dynamic processes in cells and organisms requires very fast and sensitive acquisition methods. Confocal laser scanning microscopy is inherently speed-limited by the requirement of beam scanning movements. In contrast to single beam scanning systems, the parallelized approach of multi-beam scanning is much faster. Spinning disk confocal microscopes are therefore very suited for fast in vivo imaging. The principles of spinning disk microscopy will be explained in this chapter and a thorough comparison of the performance of single beam and multi-beam scanning systems is made and illustrated with an example of in vivo imaging in Dictyostelium discoideum.
对细胞和生物体中的动态过程进行体内显微镜观察需要非常快速且灵敏的采集方法。共聚焦激光扫描显微镜由于光束扫描运动的要求而固有地存在速度限制。与单光束扫描系统不同，多光束扫描的并行化方法要快得多。因此，转盘共聚焦显微镜非常适合快速的体内成像。本章将解释转盘显微镜的原理，并对单光束和多光束扫描系统的性能进行全面比较，并以盘基网柄菌体内成像的例子进行说明。
