Sinzato Yuri Karen, Lima Paula Helena Ortiz, Santos Carlos Eduardo Meirelles dos, Campos Kleber Eduardode, Rudge Marilza Vieira Cunha, Damasceno Débora Cristina
Laboratory of Experimental Research in Gynecology and Obstetrics, Botucatu Medical School, UNESP, Brazil.
Acta Cir Bras. 2008 Nov-Dec;23(6):481-5. doi: 10.1590/s0102-86502008000600002.
To evaluate cigarette smoke exposure and/or diabetes association effects on the glycemia and liver glycogen levels of pregnant Wistar rats.
60 adult rats were randomly distributed into (n=10/group): non-diabetic exposed to filtered air (G1); non-diabetic exposed to cigarette smoke only before pregnancy (G2); non-diabetic exposed to cigarette smoke before and during pregnancy (G3); diabetic exposed to filtered air (G4); diabetic exposed to cigarette smoke only before pregnancy (G5), and diabetic exposed to cigarette smoke before and during pregnancy (G6). Glycemia was determined at days 0 and 21 of pregnancy. Liver samples were collected for liver glycogen determinations.
At day 21 of pregnancy, glycemia was higher in G5 and G6 compared to G4 group. G2 (2.43+/-0.43), G3 (3.20+/-0.49), G4 (2.62+/-0.34), G5 (2.65+/-0.27) and G6 groups (1.94+/-0.35) presented decreased liver glycogen concentrations compared to G1 (4.20+/-0.18 mg/100mg liver tissue) (p<0.05). G5 and G6 groups presented decreased maternal weight gain and litter weight.
Severe diabetes and cigarette smoke exposure, alone or associated, caused impairment in liver glycogen storage at term pregnancy. Due to the fact that liver glycogen storages were considered determinant for glucose tolerance, it is relevant to point out a rigid clinical glycemic control and to stop smoking so earlier in pregnancy programming.
评估香烟烟雾暴露和/或糖尿病对妊娠Wistar大鼠血糖和肝糖原水平的联合影响。
60只成年大鼠随机分为（每组n = 10）：暴露于过滤空气的非糖尿病组（G1）；仅在妊娠前暴露于香烟烟雾的非糖尿病组（G2）；妊娠前及妊娠期均暴露于香烟烟雾的非糖尿病组（G3）；暴露于过滤空气的糖尿病组（G4）；仅在妊娠前暴露于香烟烟雾的糖尿病组（G5）；妊娠前及妊娠期均暴露于香烟烟雾的糖尿病组（G6）。在妊娠第0天和第21天测定血糖。采集肝脏样本用于肝糖原测定。
妊娠第21天，G5组和G6组的血糖高于G4组。与G1组（4.20±0.18mg/100mg肝组织）相比，G2组（2.43±0.43）、G3组（3.20±0.49）、G4组（2.62±0.34）、G5组（2.65±0.27）和G6组（1.94±0.35）的肝糖原浓度降低（p<0.05）。G5组和G6组的母鼠体重增加和窝仔体重降低。
严重糖尿病和香烟烟雾暴露单独或联合作用，导致足月妊娠时肝糖原储存受损。鉴于肝糖原储存被认为是葡萄糖耐量的决定因素，指出在孕期早期进行严格的临床血糖控制和戒烟具有重要意义。
