Gomes Patrícia Rodrigues Lourenço, Seraphim Patricia Monteiro
Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista Júlio de Mesquista Filho, Presidente Prudente, SP, Brasil.
Rev Bras Ginecol Obstet. 2010 Dec;32(12):591-6. doi: 10.1590/s0100-72032010001200005.
to investigate the effect of cigarette smoke exposure on body and tissue weight gain, serum parameters and milk yield during pregnancy and lactation in rats, and the impact on offspring from birth toil young adulthood.
40 Wistar pregnant rats were randomly divided into: CG - not exposed to cigarette smoke and sacrificed at the end of pregnancy; CL - not exposed to cigarette smoke and sacrificed at the end of lactation; FG - exposed to cigarette smoke and sacrificed at the end of pregnancy; FL - exposed to cigarette smoke and sacrificed at the end of lactation. The offspring were separated by gender and divided according to their mothers' groups. Tissue weight, body weight and serum parameters were evaluated in rats and offspring. Milk yield per pup was calculated.
body weight was decreased in FL during lactation (CL=267.0 ± 7.2; FL=235.5 ± 7.2 g*,p<0.05). Adipose tissue was not detected in the CL and FL groups, and was reduced in FG compared to CG (CG=3.3 ± 0.3; FG=2.4 ± 0.3 g, p<0.05). Rats exposed to cigarette smoke had higher blood glucose levels (CG=113 ± 17, CL=86 ± 16, FG=177 ± 21, FL=178 ± 23 mg/dL*, p<0.05 CG versus FG e CL versus FL), CL and FL groups presented lower HDL-cholesterol with no change in total cholesterol. Finally, rats exposed to cigarette smoke had lower milk yield compared to unexposed rats (CL=6.7 ± 0.4, FL=5.4 ± 0.3 g, p<0.05). In offspring from the FG and FL groups, there was a decrease of body weight from birth to young adulthood, with no changes in gastrocnemius, liver or heart weights in any group, and adipose tissue was no detected in female offspring. There was an increase in blood glucose in offspring of both sexes from rats exposed to cigarette smoke (males: Pcg=107 ± 10.5, Pcl=115 ± 8.6, Pfg=148 ± 16.8, Pfl=172 ± 11.2**; females: Pcg=109 ± 27.2, Pcl=104 ± 9.7, Pfg=134 ± 20.0*, Pfl=126 ± 13.3**; p<0.05 *Pcg versus Pfg and **Pcl versus Pfl).
exposure to cigarette smoke provokes impairment of morphometric and serum parameters during pregnancy and lactation both in mothers and offspring, which is maintained during young adulthood.
研究孕期和哺乳期暴露于香烟烟雾对大鼠体重、组织重量增加、血清参数及产奶量的影响，以及对后代从出生到幼年的影响。
40只Wistar孕鼠随机分为：CG组——未暴露于香烟烟雾，在孕期结束时处死；CL组——未暴露于香烟烟雾，在哺乳期结束时处死；FG组——暴露于香烟烟雾，在孕期结束时处死；FL组——暴露于香烟烟雾，在哺乳期结束时处死。后代按性别分开，并根据其母鼠的分组进行划分。对大鼠及其后代的组织重量、体重和血清参数进行评估。计算每只幼崽的产奶量。
哺乳期FL组体重下降（CL = 267.0 ± 7.2；FL = 235.5 ± 7.2 g，p<0.05）。CL组和FL组未检测到脂肪组织，与CG组相比，FG组脂肪组织减少（CG = 3.3 ± 0.3；FG = 2.4 ± 0.3 g，p<0.05）。暴露于香烟烟雾的大鼠血糖水平较高（CG = 113 ± 17，CL = 86 ± 16，FG = 177 ± 21*，FL = 178 ± 23 mg/dL*，p<0.05，CG与FG对比，CL与FL对比），CL组和FL组高密度脂蛋白胆固醇较低，总胆固醇无变化。最后，与未暴露的大鼠相比，暴露于香烟烟雾的大鼠产奶量较低（CL = 6.7 ± 0.4，FL = 5.4 ± 0.3 g，p<0.05）。在FG组和FL组的后代中，从出生到幼年体重下降，任何组的腓肠肌、肝脏或心脏重量均无变化，雌性后代未检测到脂肪组织。暴露于香烟烟雾的大鼠后代两性血糖均升高（雄性：Pcg = 107 ± 10.5，Pcl = 115 ± 8.6，Pfg = 148 ± 16.8*，Pfl = 172 ± 11.2**；雌性：Pcg = 109 ± 27.2，Pcl = 104 ± 9.7，Pfg = 134 ± 20.0*，Pfl = 126 ± 13.3**；p<0.05，*Pcg与Pfg对比，*Pcl与Pfl对比）。
暴露于香烟烟雾会导致孕期和哺乳期母鼠及其后代的形态学和血清参数受损，且这种影响在幼年时仍持续存在。
