Zhanel G G, Karlowsky J A, Hoban D J, Davidson R J
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
Chemotherapy. 1991;37(2):114-21. doi: 10.1159/000238842.
This investigation used the postantibiotic effect (PAE) and killing curves to provide data on the antimicrobial activity of subinhibitory (1/8x, 1/4x and 1/2x minimum inhibitory concentration; MIC) and inhibitory (1x MIC) concentrations of amikacin, gentamicin and tobramycin against Pseudomonas aeruginosa. Subinhibitory concentrations (1/4x and 1/2x MIC) of aminoglycosides demonstrated a reproducible PAE. At 1/4x MIC, the order of duration of the PAE was approximately 15 min for all aminoglycosides, while at 1/2x MIC all three aminoglycosides displayed a similar PAE of approximately 40 min. Killing-curve studies demonstrated that subinhibitory concentrations of aminoglycosides either decrease bacterial growth for several hours (1/4x and 1/2x MIC) or produce stasis of growth for several hours (1/8x MIC). Only inhibitory aminoglycoside concentrations (1x MIC) proved to be bactericidal. Subinhibitory concentrations of aminoglycosides decrease bacterial growth and produce a PAE against P. aeruginosa.
本研究采用抗生素后效应（PAE）和杀菌曲线，以提供关于阿米卡星、庆大霉素和妥布霉素对铜绿假单胞菌的亚抑菌浓度（1/8倍、1/4倍和1/2倍最低抑菌浓度；MIC）及抑菌浓度（1倍MIC）抗菌活性的数据。氨基糖苷类药物的亚抑菌浓度（1/4倍和1/2倍MIC）显示出可重复的PAE。在1/4倍MIC时，所有氨基糖苷类药物的PAE持续时间约为15分钟，而在1/2倍MIC时，所有三种氨基糖苷类药物的PAE相似，约为40分钟。杀菌曲线研究表明，氨基糖苷类药物的亚抑菌浓度要么使细菌生长减缓数小时（1/4倍和1/2倍MIC），要么使生长停滞数小时（1/8倍MIC）。只有抑菌浓度的氨基糖苷类药物（1倍MIC）被证明具有杀菌作用。氨基糖苷类药物的亚抑菌浓度可减缓铜绿假单胞菌的生长并产生PAE。
