黄腐酚影响前脂肪细胞分化：抗增殖和凋亡作用的影响

Xanthohumol influences preadipocyte differentiation: implication of antiproliferative and apoptotic effects.

作者信息

Mendes Vanda, Monteiro Rosário, Pestana Diogo, Teixeira Diana, Calhau Conceição, Azevedo Isabel

机构信息

Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Al. Prof. Hernani Monteiro, 4200-319 Porto, Portugal.

出版信息

J Agric Food Chem. 2008 Dec 24;56(24):11631-7. doi: 10.1021/jf802233q.

DOI:10.1021/jf802233q

PMID:19035642

Abstract

There is interest in the research of natural compounds that may interfere with the adipocyte life cycle, due to the growing prevalence of obesity and related complications. We aimed at studying the effect of xanthohumol (XN), a Humulus lupulus L. prenylflavonoid, on adipocytes measuring differentiation, proliferation, and apoptosis in 3T3-L1 cells. XN reduced differentiation, as revealed by decreased lipid content and peroxisome proliferator-activated receptor gamma expression, an effect more pronounced when cells were treated before or during differentiation induction. XN also decreased proliferation, as measured by sulforhodamine staining (IC(50) between 26 and 12 microM for 24, 48, and 72 h), and preadipocyte Ki67 expression. Apoptosis was increased in preadipocytes and adipocytes. NF-kappaB activity was stimulated by XN in preadipocytes. Results suggest that XN may reduce adipocyte number, contributing to adipocyte hypertrophy. Taking into consideration the consequences of adipocyte hypertrophy, XN does not seem to improve the metabolic profile associated with obesity.

摘要

由于肥胖症及相关并发症的患病率不断上升，人们对可能干扰脂肪细胞生命周期的天然化合物研究产生了兴趣。我们旨在研究啤酒花 prenylflavonoid 化合物黄腐酚（XN）对 3T3-L1 细胞中脂肪细胞分化、增殖和凋亡的影响。XN 降低了分化，这表现为脂质含量和过氧化物酶体增殖物激活受体γ表达的降低，当细胞在分化诱导之前或期间接受处理时，这种效果更为明显。XN 还降低了增殖，通过磺酰罗丹明染色测量（24、48 和 72 小时的 IC(50)在 26 至 12 μM 之间）以及前脂肪细胞 Ki67 表达。前脂肪细胞和脂肪细胞中的凋亡增加。XN 在脂肪前体细胞中刺激了 NF-κB 活性。结果表明，XN 可能会减少脂肪细胞数量，导致脂肪细胞肥大。考虑到脂肪细胞肥大的后果，XN 似乎并不能改善与肥胖相关的代谢状况。