Tsionkis Georgios, Andronidou Elisavet M, Kontou Panagiota I, Tamposis Ioannis A, Tegopoulos Konstantinos, Pergantas Panagiotis, Grigoriou Maria E, Skavdis George, Bagos Pantelis G, Braliou Georgia G
Department of Computer Science and Biomedical Informatics, University of Thessaly, 35131 Lamia, Greece.
Department of Mathematics, University of Thessaly, 35132 Lamia, Greece.
Pharmaceuticals (Basel). 2025 Jul 31;18(8):1139. doi: 10.3390/ph18081139.
: (hops) are a perennial, dioecious plant widely cultivated for beer production, used for their distinguishing aroma and bitterness-traits that confer high added value status. Various hop-derived compounds have been reported to exhibit antioxidant, antimicrobial, antiproliferative and other bioactive effects. This systematic review and meta-analysis assesses the impact of hop compounds on the viability of diverse cancer cell lines. : A comprehensive literature search was performed following PRISMA guidelines. Data were synthesized via multivariate meta-analysis and meta-regression, using IC values as the effect size. Key variables included assay type (SRB, tetrazolium salt-based, crystal violet), exposure duration (24, 48, 72 h), specific hop compound and cancer cell line. : Of 622 articles identified, 61 met eligibility criteria, yielding 354 individual experiments. Meta-regression of xanthohumol (XN) IC values across SRB, tetrazolium and crystal violet assays revealed no statistically significant differences at 24 h ( = 0.77), 48 h ( = 0.35) and 72 h ( = 0.70), supporting the interchangeability of the methods. Meta-analysis confirmed that hop constituents inhibit cancer cell proliferation; XN emerged as the most potent flavonoid (IC = 16.89 μM at 72 h), while lupulone was the strongest compound overall (IC = 5.00 μM at 72 h). Crude hop extracts demonstrated greater antiproliferative selectivity for cancer versus non-cancer cells (IC = 35.23 vs. 43.80 μg/mL at 72 h). : Hop compounds, and particularly bitter acids, demonstrate promising antiproliferative activity against cancer cells with comparatively low toxicity to healthy cells. Furthermore, our analysis confirms the comparability of SRB, tetrazolium-based and crystal violet assays, supporting the robust integration of antiproliferative data.
啤酒花是一种多年生雌雄异株植物，因其独特的香气和苦味特性而被广泛种植用于啤酒生产，具有很高的附加值。据报道，各种啤酒花衍生化合物具有抗氧化、抗菌、抗增殖和其他生物活性作用。本系统评价和荟萃分析评估了啤酒花化合物对多种癌细胞系活力的影响。：按照PRISMA指南进行了全面的文献检索。通过多变量荟萃分析和荟萃回归合成数据，使用IC值作为效应量。关键变量包括检测类型（SRB法、基于四唑盐的方法、结晶紫法）、暴露持续时间（24、48、72小时）、特定的啤酒花化合物和癌细胞系。：在鉴定出的622篇文章中，61篇符合纳入标准，产生了354个独立实验。对黄腐酚（XN）在SRB法、四唑盐法和结晶紫法中的IC值进行荟萃回归分析，发现在24小时（P = 0.77）、48小时（P = 0.35）和72小时（P = 0.70）时无统计学显著差异，支持这些方法的互换性。荟萃分析证实啤酒花成分可抑制癌细胞增殖；XN是最有效的黄酮类化合物（72小时时IC = 16.89 μM），而蛇麻酮总体上是最强的化合物（72小时时IC = 5.00 μM）。粗啤酒花提取物对癌细胞的抗增殖选择性高于对非癌细胞（72小时时IC = 35.23 vs. 43.80 μg/mL）。：啤酒花化合物，尤其是苦味酸，对癌细胞显示出有前景的抗增殖活性，对健康细胞的毒性相对较低。此外，我们的分析证实了SRB法、基于四唑盐的方法和结晶紫法的可比性，支持抗增殖数据的可靠整合。
