Kokoza V A, Baricheva E M, Katokhin A V
Genetika. 1991 Jan;27(1):51-60.
Screening of Drosophila melanogaster genomic library was carried out using mouse brain polysomal poly(A)+RNA. As a result, 100 clones were selected, among which 14 clones were picked up after hybridization with fly head poly(A)+RNA. It follows therefore, that these clones contain evolutionary conserved sequences which are expressed in Drosophila fly heads. Analysis of these 14 clones revealed RNA-coding fragments. Comparison of their expression in heads and bodies of Drosophila was carried out. Using in situ hybridization we determined the localization of selected 14 sequences on polytene chromosomes. The possibility of further analysis of some clones to study developmental and functional processes in neural system of Drosophila is discussed.
利用小鼠脑多核糖体多聚腺苷酸加尾RNA对黑腹果蝇基因组文库进行筛选。结果，挑选出100个克隆，其中14个克隆在与果蝇头部多聚腺苷酸加尾RNA杂交后被挑选出来。因此可以推断，这些克隆包含在果蝇头部表达的进化保守序列。对这14个克隆的分析揭示了RNA编码片段。对它们在果蝇头部和身体中的表达进行了比较。利用原位杂交技术，我们确定了所选14个序列在多线染色体上的定位。讨论了进一步分析一些克隆以研究果蝇神经系统发育和功能过程的可能性。
