Nielson Rex, Kaehr Bryan, Shear Jason B
Department of Chemistry & Biochemistry, 1 University Station A5300, University of Texas, Austin, TX 78712, USA.
Small. 2009 Jan;5(1):120-5. doi: 10.1002/smll.200801084.
A strategy for rapidly printing three-dimensional (3D) microscopic replicas using multiphoton lithography directed by a dynamic electronic mask is reported. Morphological descriptions of 3D structures are encoded as stacks of 2D slices created from tomographic and computer-designed instruction sets. In this manner, digital images serve as input for a sequence of reflective photomasks on a digital micromirror device to direct replication of a structure. By scanning a laser focus across the face of the intrinsically aligned masks, tomographic and computed data can be translated into protein-based 3D reproductions with submicrometer feature sizes within 1 min. This straightforward and highly versatile approach may provide improved routes for the development of 3D cellular scaffolds, rapid prototyping of microanalytical devices, and production of custom tissue replacements.
报道了一种使用由动态电子掩膜引导的多光子光刻技术快速打印三维（3D）微观复制品的策略。3D结构的形态描述被编码为由断层扫描和计算机设计指令集创建的2D切片堆栈。通过这种方式，数字图像作为数字微镜器件上一系列反射光掩膜的输入，以指导结构的复制。通过在本质上对齐的掩膜表面扫描激光焦点，断层扫描和计算数据可以在1分钟内转化为具有亚微米特征尺寸的基于蛋白质的3D复制品。这种直接且高度通用的方法可能为3D细胞支架的开发、微分析设备的快速原型制作以及定制组织替代物的生产提供改进的途径。
