Wright M S, Eckert J J, Biel S W, Biel A J
Department of Microbiology, Louisiana State University, Baton Rouge 70803.
FEMS Microbiol Lett. 1991 Mar 1;62(2-3):339-42. doi: 10.1016/0378-1097(91)90181-9.
An EcoRI fragment containing the Rhodobacter capsulatus hemA promoter has been cloned into a lacZ translational fusion vector. The resulting plasmid produced a hemA-lacZ fusion protein with a molecular mass of 147,000. Expression of the hemA-lacZ fusion, as measured by production of beta-galactosidase, was regulated 2- to 3-fold by oxygen tension. The unexpectedly small change in beta-galactosidase levels suggests that transcriptional regulation of the hemA gene is not the major factor in oxygen-mediated control of porphyrin synthesis.
