Henle W, Henie G
J Natl Cancer Inst. 1977 Mar;58(3):785-6. doi: 10.1093/jnci/58.3.785.
African patients with Burkitt's lymphoma (BL) may show antibodies to the R (restricted) component of the Epstein-Barr (EB) virus-induced early antigens that appear to match in titer the antibodies to EB viral capsid antigen (VCA) as determined with the aid of acetone-fixed smears of EB3 cells. Virus-producing EB3 cells, however, contain the R component as well as VCA so that the correct anti-VCA titers in such cases remain in doubt. Since the R component, but not VCA, was found to be denatured by methanol fixation, the parallel use of acetone- and methanol-fixed EB3 cell smears permitted the determination of the correct anti-VCA titer. Our results showed that the anti-R titers of BL patients can in fact be up to eightfold higher than the anti-VCA titers. Anti-R titers equaling or exceeding anti-VCA titers have been encountered thus far only in BL.
患有伯基特淋巴瘤（BL）的非洲患者可能会表现出针对爱泼斯坦 - 巴尔（EB）病毒诱导的早期抗原的R（受限）成分的抗体，借助EB3细胞的丙酮固定涂片测定，这些抗体的滴度似乎与针对EB病毒衣壳抗原（VCA）的抗体滴度相匹配。然而，产生病毒的EB3细胞同时含有R成分和VCA，因此在这种情况下正确的抗VCA滴度仍存在疑问。由于发现R成分可被甲醇固定变性，而VCA不会，同时使用丙酮固定和甲醇固定的EB3细胞涂片可确定正确的抗VCA滴度。我们的结果表明，BL患者的抗R滴度实际上可能比抗VCA滴度高八倍。到目前为止，仅在BL患者中发现抗R滴度等于或超过抗VCA滴度的情况。
