Sezer Ali Demir, Cevher Erdal, Hatipoğlu Fatih, Oğurtan Zeki, Baş Ahmet Levent, Akbuğa Jülide
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Marmara University, Istanbul, Turkey.
Biol Pharm Bull. 2008 Dec;31(12):2326-33. doi: 10.1248/bpb.31.2326.
Treatment of dermal wounds with macromolecular agents such as natural polymers is one of the research areas of the biomaterial science. Fucoidan is a sulphated polysaccharide which is commonly obtained from seaweeds. The great number of studies on the different pharmacological properties of fucoidan is present, but there is limited information about using of fucoidan in the treatment of dermal burns. The aim of this study was to prepare fucoidan-chitosan hydrogels and to investigate their treatment efficiency on dermal burns. Hydrogels were prepared by swelling the polymers in acidic solution and their swelling, mechanical (hardness, cohesiveness and adhesiveness) and bioadhesive properties were investigated. The viscosity and water absorption capacity of formulations increased with increase in the polymer concentration. In contrast to the cohesiveness results, the adhesiveness of hydrogels increased with the polymer concentration. The bioadhesion was changed between 0.012-0.142 mJ x cm(-2) and enhanced with addition of fucoidan into gel formulations. It was formed dermal burns on seven adult male New Zealand white rabbits and the optimum gel formulation applied on the wounds. Control and treatment group biopsy samples were taken on days 7, 14 and 21 and each burn wound site was evaluated histopathologically. No edema was seen in tested groups except control after 3 d treatment. After 7 d treatment, fibroplasia and scar were fixed on wounds treated with fucoidan-chitosan gel and fucoidan solution. The best regeneration on dermal papillary formation and the fastest closure of the wounds were observed in fucoidan-chitosan hydrogels after 14 d treatment.
使用天然聚合物等大分子试剂治疗皮肤伤口是生物材料科学的研究领域之一。岩藻依聚糖是一种通常从海藻中提取的硫酸化多糖。目前有大量关于岩藻依聚糖不同药理特性的研究，但关于其在治疗皮肤烧伤方面的应用信息有限。本研究的目的是制备岩藻依聚糖 - 壳聚糖水凝胶，并研究其对皮肤烧伤的治疗效果。通过在酸性溶液中溶胀聚合物来制备水凝胶，并研究其溶胀、机械性能（硬度、内聚性和粘附性）及生物粘附性能。制剂的粘度和吸水能力随聚合物浓度的增加而增加。与内聚性结果相反，水凝胶的粘附性随聚合物浓度的增加而增加。生物粘附力在0.012 - 0.142 mJ·cm⁻²之间变化，并随着向凝胶制剂中添加岩藻依聚糖而增强。在7只成年雄性新西兰白兔身上造成皮肤烧伤，并将最佳凝胶制剂应用于伤口。在第7天、14天和21天采集对照组和治疗组的活检样本，并对每个烧伤伤口部位进行组织病理学评估。治疗3天后，除对照组外，测试组未见水肿。治疗7天后，用岩藻依聚糖 - 壳聚糖凝胶和岩藻依聚糖溶液处理的伤口出现纤维增生和瘢痕。治疗14天后，在岩藻依聚糖 - 壳聚糖水凝胶中观察到皮肤乳头形成的最佳再生和伤口的最快愈合。
