Yamane T, Fujita Y, Sagara Y, Takahashi T, Imanishi J
First Department of Surgery, Kyoto Prefectural University of Medicine.
Jpn J Cancer Res. 1991 Apr;82(4):470-5. doi: 10.1111/j.1349-7006.1991.tb01872.x.
The inhibitory effect of murine interferon alpha/beta (Mu-IFN) on the induction of adenocarcinoma of the duodenum in C57BL/6 mice given N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) was examined. ENNG was given continuously in drinking water for 4 weeks and Mu-IFN was given intraperitoneally for 12 weeks. Then, the duodenal tumors of mice were examined stereomicroscopically and histologically. The level of IFN activity and natural killer (NK) activity were evaluated after an intraperitoneal single injection of Mu-IFN, and the level of NK activity was evaluated 2, 5 and 8 weeks after giving ENNG and Mu-IFN. In the mice given Mu-IFN, the incidence of duodenal tumors was significantly decreased (P less than 0.01), compared with that in mice given ENNG alone. Further, anti-asialo GM1 was given intraperitoneally every 5 days for 8 weeks to depress NK function and the incidence and size of duodenal tumors were examined. The results indicated that NK cells also have an important effect on the process of carcinogenesis. These data suggest that chemoprevention with IFN may be feasible.
研究了小鼠α/β干扰素（Mu-IFN）对给予N-乙基-N'-硝基-N-亚硝基胍（ENNG）的C57BL/6小鼠十二指肠腺癌诱导的抑制作用。ENNG连续4周给予饮用水，Mu-IFN腹腔注射12周。然后，对小鼠的十二指肠肿瘤进行立体显微镜和组织学检查。腹腔单次注射Mu-IFN后评估IFN活性和自然杀伤（NK）活性水平，并在给予ENNG和Mu-IFN后2、5和8周评估NK活性水平。与仅给予ENNG的小鼠相比，给予Mu-IFN的小鼠十二指肠肿瘤发生率显著降低（P<0.01）。此外，每5天腹腔注射抗唾液酸GM1，持续8周以抑制NK功能，并检查十二指肠肿瘤的发生率和大小。结果表明，NK细胞在致癌过程中也具有重要作用。这些数据表明，用IFN进行化学预防可能是可行的。
