沃氏甲烷球菌中辅酶M营养缺陷型突变体的分离及电穿孔转化
Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae.
作者信息
Micheletti P A, Sment K A, Konisky J
机构信息
Department of Microbiology, University of Illinois, Urbana 61801.
出版信息
J Bacteriol. 1991 Jun;173(11):3414-8. doi: 10.1128/jb.173.11.3414-3418.1991.
Abstract
An auxotrophic mutant of Methanococcus voltae was isolated that required coenzyme M (CoM) for growth. With the mutant as a recipient, conditions were developed that allowed the introduction of wild-type CoM+ DNA into the mutant methanogen via electroporation. This method also allowed the rescue of both a histidine and purine auxotroph as well as the introduction of DNA determining resistance to the CoM analog 2-bromoethanesulfonic acid. Electroporation of the CoM(+)-determining DNA was 50- to 80-fold more efficient than natural transformation.
摘要
分离出了沃氏甲烷球菌的一种营养缺陷型突变体，其生长需要辅酶M（CoM）。以该突变体作为受体，开发了一些条件，使得野生型CoM⁺ DNA能够通过电穿孔导入突变产甲烷菌中。这种方法还能挽救组氨酸和嘌呤营养缺陷型，以及导入决定对CoM类似物2-溴乙烷磺酸抗性的DNA。决定CoM⁺的DNA的电穿孔效率比自然转化高50至80倍。
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10
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