嗜热自养甲烷杆菌马尔堡菌株中的遗传转化系统。
Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg.
作者信息
Worrell V E, Nagle D P, McCarthy D, Eisenbraun A
机构信息
Department of Botany and Microbiology, University of Oklahoma, Norman 73019.
出版信息
J Bacteriol. 1988 Feb;170(2):653-6. doi: 10.1128/jb.170.2.653-656.1988.
Abstract
A wild-type strain of Methanobacterium thermoautotrophicum Marburg was transformed by DNA from strains resistant to 5-fluorouracil. Recipient cells were grown without selection on gellan gum (GELRITE) plates with DNA. Drug-resistant cells were recovered by replica plating the resulting colonies onto drug plates. Transformation required high-molecular-weight DNA with appropriate markers and was not observed on agar or in liquid media under a variety of conditions.
摘要
嗜热自养甲烷杆菌马尔堡野生型菌株被来自对5-氟尿嘧啶有抗性的菌株的DNA转化。受体细胞在含有DNA的结冷胶（吉兰糖胶）平板上不进行选择培养。通过将所得菌落影印接种到含药平板上回收耐药细胞。转化需要具有适当标记的高分子量DNA，并且在各种条件下在琼脂或液体培养基中均未观察到转化现象。
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