Nakajima S, Sakata I, Omote Y, Yamazaki K, Maeda T, Kubo Y, Samejima N, Takemura T, Shindo Y, Yamauchi H
Division of Surgical Operation, Asahikawa Medical College, Japan.
J Photochem Photobiol B. 1991 Mar;8(4):409-17. doi: 10.1016/1011-1344(91)80115-x.
Spectrofluorometry, radioisotope (RI) labelling and high performance liquid chromatography (HPLC) can be used to estimate photosensitizer concentrations. The biodistribution of porphyrins and metalloporphyrins containing the bifunctional chelating agent diethylenetriamine pentaacetic acid (DTPA) was examined in tumour-bearing mice by nitrogen-pulsed laser spectrofluorometry (PLS) and RI labelling. The biodistribution of metalloporphyrin amino acid derivatives containing alkoxyl groups was also examined by PLS and HPLC analysis using an acetone powder extraction method. Spectrofluorometry is useful for estimating the biodistribution of porphyrins in tumour, lung, kidney and serum, but not in liver. However, spectrofluorometry cannot be used to evaluate the concentration of certain metalloporphyrins such as manganese complexes. The concentrations of porphyrins in liver measured by PLS and two other methods showed remarkable differences. RI labelling and HPLC analysis are obviously tedious methods. Therefore it seems practical to screen for a number of compounds using the spectrofluorometric method (PLS). Subsequently, the porphyrins which give good results with PLS should be measured using RI labelling and HPLC.
荧光分光光度法、放射性同位素（RI）标记法和高效液相色谱法（HPLC）可用于估算光敏剂浓度。采用氮脉冲激光荧光分光光度法（PLS）和RI标记法，研究了含双功能螯合剂二乙烯三胺五乙酸（DTPA）的卟啉和金属卟啉在荷瘤小鼠体内的生物分布。还通过PLS和使用丙酮粉提取法的HPLC分析，研究了含烷氧基的金属卟啉氨基酸衍生物的生物分布。荧光分光光度法有助于估算卟啉在肿瘤、肺、肾和血清中的生物分布，但对肝脏则不适用。然而，荧光分光光度法不能用于评估某些金属卟啉（如锰配合物）的浓度。通过PLS和其他两种方法测得的肝脏中卟啉浓度显示出显著差异。RI标记法和HPLC分析显然是繁琐的方法。因此，使用荧光分光光度法（PLS）筛选大量化合物似乎是可行的。随后，对于用PLS得到良好结果的卟啉，应采用RI标记法和HPLC进行测量。
