Justice Bradley A, Badr Nadia A, Felder Robin A
Global Cell Solutions, Inc., 770 Harris Street, Suite 104, Charlottesville, VA 22903, USA.
Drug Discov Today. 2009 Jan;14(1-2):102-7. doi: 10.1016/j.drudis.2008.11.006. Epub 2008 Dec 26.
3D cell culture technologies have revolutionized our understanding of cellular behavior, both in culture and in vivo, but adoption by cell-based screening groups has been slow owing to problems of consistency, scale and cost. The evolving field of high content screening technologies will, however, require a rethinking of 3D cell culture adoption to ensure the next generation of cells provide relevant in vivo-like data. Three current technologies are presented in this review: membranes, sponges/gels and microcarriers. A short history of these technologies and unique research applications are discussed. Also, the technologies are evaluated for usefulness in modern automated cell-based screening equipment.
3D细胞培养技术彻底改变了我们对细胞在培养和体内行为的理解，但由于一致性、规模和成本等问题，基于细胞的筛选团队对其采用一直很缓慢。然而，不断发展的高内涵筛选技术领域将需要重新思考3D细胞培养的采用，以确保下一代细胞能提供类似体内的相关数据。本文综述了三种当前技术：膜、海绵/凝胶和微载体。讨论了这些技术的简短历史和独特的研究应用。此外，还评估了这些技术在现代基于细胞的自动化筛选设备中的实用性。
