Godbole Nihar, Lai Andrew, Carrion Flavio, Scholz-Romero Katherin, Ravichandran Akhilandeshwari, Kalita-de Croft Priyakshi, McCart Reed Amy E, Joshi Vaibhavi, Lakhani Sunil R, Masud Mostafa Kamal, Yamauchi Yusuke, Perrin Lewis, Hooper John, Bray Laura, Guanzon Dominic, Salomon Carlos
Translational Extracellular Vesicles in Obstetrics and Gynae-Oncology Group, Centre for Clinical Diagnostics, UQ Centre for Clinical Research (UQCCR), Royal Brisbane and Women's Hospital, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia.
UQ Centre for Extracellular Nanomedicine, Faculty of Medicine, The University of Queensland, Herston, QLD 4029, Australia.
Heliyon. 2025 Jan 23;11(4):e42188. doi: 10.1016/j.heliyon.2025.e42188. eCollection 2025 Feb 28.
Ovarian cancer is the most common gynaecological malignancy and the seventh most diagnosed cancer in females worldwide. Currently, it is the sixth leading cause of cancer related mortality among patients globally. The heterogenous origin of the disease and unambiguous nature of the clinical symptoms leading to delayed detection has been one of the key reasons for increasing mortality. Hence new approaches are required to understand the biology of ovarian cancer, where the use of cell culture models that mimic the physiology of the disease is fundamental. Cell culture serves as a crucial in vitro tool, contributing to our comprehension of various aspects of cell biology, tissue morphology, disease mechanisms, drug responses, protein production, and tissue engineering. A significant portion of in vitro studies rely on two-dimensional (2D) cell cultures, however, these cultures present notable limitations, for example disruptions in cellular and extracellular interactions, alterations in cell morphology, polarity, and division mechanisms. Recently, extracellular vesicles have been identified as crucial players in cell biology as part of the communication system that cancer cells use to metastasize. We optimized and compared three-dimensional (3D) culture of ovarian cancer cells lines (SKOV-3 and OVCAR-3) with two-dimensional models based on their protein and miRNA content. We further investigated whether extracellular vesicles from these models reflect changes in cancer cells, and aid in the identification of overall survival in women with ovarian cancer.
卵巢癌是最常见的妇科恶性肿瘤，也是全球女性中第七大最常被诊断出的癌症。目前，它是全球癌症相关死亡的第六大主要原因。该疾病起源的异质性以及导致检测延迟的临床症状的不明确性一直是死亡率上升的关键原因之一。因此，需要新的方法来了解卵巢癌的生物学特性，其中使用模拟该疾病生理学的细胞培养模型至关重要。细胞培养是一种关键的体外工具，有助于我们理解细胞生物学、组织形态学、疾病机制、药物反应、蛋白质生产和组织工程的各个方面。很大一部分体外研究依赖于二维（2D）细胞培养，然而，这些培养存在明显的局限性，例如细胞与细胞外相互作用的破坏、细胞形态、极性和分裂机制的改变。最近，细胞外囊泡已被确定为细胞生物学中的关键参与者，是癌细胞用于转移的通讯系统的一部分。我们基于蛋白质和miRNA含量，对卵巢癌细胞系（SKOV-3和OVCAR-3）的三维（3D）培养与二维模型进行了优化和比较。我们进一步研究了来自这些模型的细胞外囊泡是否反映癌细胞的变化，并有助于确定卵巢癌女性的总生存期。
