Blanchard D K, Michelini-Norris M B, Djeu J Y
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612.
J Infect Dis. 1991 Jul;164(1):152-7. doi: 10.1093/infdis/164.1.152.
Using a rapid radiolabel assay, monocytes derived from the peripheral blood of normal donors were found to kill 40%-92% of inoculated Mycobacterium avium-intracellulare complex (MAC), an opportunistic pathogen commonly found in AIDS patients. However, bactericidal activity was significantly lower in 4-day culture-derived macrophages compared with matched monocyte cultures. The addition of interferon-gamma (IFN-gamma) to monocytes was found to inhibit the bactericidal activity of fresh monocytes. The number of bacteria recovered from fresh monocytes exposed to IFN-gamma was significantly higher than that in control cultures with MAC alone, suggesting that intracellular MAC growth could be stimulated by IFN-gamma. This enhancement of MAC survival and growth by IFN-gamma was not observed when culture-derived macrophages were used. Similar results were obtained with IFN-alpha/A2. These results indicate, therefore, that the innate efficiency of mycobacterial killing by monocytes can be down-regulated by IFN, but macrophages are not significantly affected.
通过快速放射性标记测定法发现，来自正常供体外周血的单核细胞能够杀死40%-92%接种的鸟分枝杆菌-胞内分枝杆菌复合体（MAC），这是一种在艾滋病患者中常见的机会性病原体。然而，与匹配的单核细胞培养物相比，4天培养的巨噬细胞的杀菌活性显著降低。向单核细胞中添加干扰素-γ（IFN-γ）被发现会抑制新鲜单核细胞的杀菌活性。从暴露于IFN-γ的新鲜单核细胞中回收的细菌数量显著高于仅含MAC的对照培养物中的细菌数量，这表明IFN-γ可刺激细胞内MAC的生长。当使用培养的巨噬细胞时，未观察到IFN-γ对MAC存活和生长的这种增强作用。使用IFN-α/A2也获得了类似结果。因此，这些结果表明，单核细胞杀灭分枝杆菌的固有效率可被IFN下调，但巨噬细胞未受到显著影响。
