Wagner Anika E, Huebbe Patricia, Konishi Tetsuya, Rahman M Mamunur, Nakahara Meiko, Matsugo Seiichi, Rimbach Gerald
Institute of Human Nutrition and Food Science, Christian-Albrechts-University Kiel, Hermann-Rodewald-Strasse 6, 24118 Kiel, Germany.
J Agric Food Chem. 2008 Dec 24;56(24):11694-9. doi: 10.1021/jf802403d.
Ascorbigen (ABG) is formed by indole-3-carbinole and ascorbic acid in brassica vegetables. In the present study, ABG has been systematically analyzed for its free radical scavenging and antioxidant capacity. To directly measure the free scavenging activity of ABG and ascorbic acid (used as a positive control), electron spin resonance and spin trapping experiments were performed. Ascorbic acid exhibited a potent free radical scavenging activity, whereas ABG did not scavenge superoxide anion free radicals and showed only little scavenging activity toward 2,2-diphenyl-1-picrylhydrazyl free radicals. Similar data were obtained for the ferric reducing ability of plasma and trolox equivalent antioxidant capacity assays. In cultured human keratinocytes, ABG counteracted tert-buthylhydroperoxide-induced cytotoxicity, whereas ascorbic acid did not exhibit any protective activity. Furthermore, in ABG-treated human keratinocytes, a decrease in tert-buthylhydroperoxide-induced lipid peroxidation was detected, whereas an ascorbic acid pretreatment did not result in the prevention of lipid peroxidation. These data indicate that ascorbic acid seems to be a more potent free radical scavenger than ABG in vitro, while ABG prevented tert-buthylhydroperoxide cytotoxicity more effectively as compared to ascorbic acid in cultured cells.
抗坏血酸原（ABG）由芸苔属蔬菜中的吲哚 - 3 - 甲醇和抗坏血酸形成。在本研究中，已对ABG的自由基清除和抗氧化能力进行了系统分析。为了直接测量ABG和抗坏血酸（用作阳性对照）的自由基清除活性，进行了电子自旋共振和自旋捕获实验。抗坏血酸表现出强大的自由基清除活性，而ABG不清除超氧阴离子自由基，对2,2 - 二苯基 - 1 - 苦基肼自由基仅表现出微弱的清除活性。在血浆铁还原能力和Trolox等效抗氧化能力测定中也获得了类似的数据。在培养的人角质形成细胞中，ABG抵消了叔丁基过氧化氢诱导的细胞毒性，而抗坏血酸未表现出任何保护活性。此外，在ABG处理的人角质形成细胞中，检测到叔丁基过氧化氢诱导的脂质过氧化减少，而抗坏血酸预处理并未导致脂质过氧化的预防。这些数据表明，在体外抗坏血酸似乎比ABG是更有效的自由基清除剂，而在培养细胞中与抗坏血酸相比ABG更有效地预防了叔丁基过氧化氢的细胞毒性。
