Lumikari M, Soukka T, Nurmio S, Tenovuo J
Department of Cariology, University of Turku, Finland.
Arch Oral Biol. 1991;36(2):155-60. doi: 10.1016/0003-9969(91)90078-9.
Streptococcus mutans, Strep. sobrinus and Lactobacillus casei were grown in glucose-supplemented, sterilized, human whole saliva, adjusted to pH 5, 6 or 7. Components of the antibacterial peroxidase system--hypothiocyanous acid (HOSCN) and hypothiocyanite ions (OSCN-)--were generated by adding exogenous H2O2 to sterilized saliva containing endogenous peroxidases and thiocyanate (SCN-) ions. HOSCN/OSCN- generation was proportional to the amount of H2O2 added, and more HOSCN/OSCN- was detected in saliva at pH 7 than at pH 5. However, the growth of mutans streptococci and L. casei was inhibited at pH 5 by HOSCN/OSCN-, whereas no inhibition was found at pH 7. The findings show that (a) sufficient amount of HOSCN/OSCN- will inhibit the growth of cariogenic bacteria in human saliva at pH 5; (b) this amount of HOSCN/OSCN- can be generated in saliva by exogenously added H2O2; and (c) peroxidase systems have stronger antistreptococcal effects in human whole saliva than in phosphate buffer.
变形链球菌、远缘链球菌和干酪乳杆菌在补充了葡萄糖、经过灭菌处理、pH值调至5、6或7的人全唾液中培养。通过向含有内源性过氧化物酶和硫氰酸盐（SCN-）离子的灭菌唾液中添加外源性过氧化氢来生成抗菌过氧化物酶系统的成分——次硫氰酸（HOSCN）和次硫氰酸根离子（OSCN-）。HOSCN/OSCN-的生成与添加的过氧化氢量成正比，并且在pH值为7的唾液中检测到的HOSCN/OSCN-比在pH值为5时更多。然而，在pH值为5时，变形链球菌和干酪乳杆菌的生长受到HOSCN/OSCN-的抑制，而在pH值为7时未发现抑制作用。研究结果表明：（a）足够量的HOSCN/OSCN-会在pH值为5的人唾液中抑制致龋菌的生长；（b）通过外源性添加过氧化氢可在唾液中生成该量的HOSCN/OSCN-；（c）过氧化物酶系统在人全唾液中比在磷酸盐缓冲液中具有更强的抗链球菌作用。
