Mansson-Rahemtulla B, Baldone D C, Pruitt K M, Rahemtulla F
J Dent Res. 1987 Feb;66(2):486-91. doi: 10.1177/00220345870660021701.
Hypothiocyanous acid (HOSCN) and hypothiocyanite (OSCN-) were generated by the antibody-independent salivary peroxidase (SP) system. The metabolism of Streptococcus mutans NCTC 10449 was examined by uniformly labeled glucose incorporation studies. We found that the SP-system causes a pH-dependent inhibition of 14C-labeled glucose uptake, and that the effects of HOSCN/OSCN- are bacteriostatic. The results also showed that, at low pH, bacteria required more time to recover fully from HOSCN/OSCN- inhibition. When control experiments were performed in the absence of HOSCN/OSCN-, but the pH was varied, we found a positive correlation between pH and the rate of 14C-glucose incorporation. The results also showed that pH did not affect the maximum incorporation of 14C-glucose, demonstrating that S. mutans can adapt to pH changes in the environment. Based on the data obtained, we postulate that the antibody-independent SP system plays an important role in the regulation of the metabolism of oral streptococci.
次硫氰酸（HOSCN）和次硫氰酸盐（OSCN-）由非抗体依赖性唾液过氧化物酶（SP）系统产生。通过均匀标记的葡萄糖掺入研究来检测变形链球菌NCTC 10449的代谢。我们发现SP系统会导致pH依赖性抑制14C标记的葡萄糖摄取，并且HOSCN/OSCN-的作用具有抑菌性。结果还表明，在低pH值下，细菌需要更多时间才能从HOSCN/OSCN-抑制中完全恢复。当在不存在HOSCN/OSCN-的情况下进行对照实验，但改变pH值时，我们发现pH值与14C-葡萄糖掺入率之间存在正相关。结果还表明，pH值不影响14C-葡萄糖的最大掺入量，这表明变形链球菌可以适应环境中的pH变化。根据获得的数据，我们推测非抗体依赖性SP系统在调节口腔链球菌代谢中起重要作用。
