Loimaranta V, Tenovuo J, Korhonen H
Institute of Dentistry, University of Turku, Finland.
Oral Microbiol Immunol. 1998 Dec;13(6):378-81. doi: 10.1111/j.1399-302x.1998.tb00695.x.
Immune whey product was obtained from Streptococcus mutans- and Streptococcus sobrinus-immunized cows. Hypothiocyanite (HOSCN/OSCN-) was generated in VMG-buffer, pH 5.5 or 6.5, by bovine milk lactoperoxidase, KSCN and hydrogen peroxide. The glucose incorporation by late-log cells of S. mutans 10449, serotype c, was followed by measuring the uptake of 14C-glucose at 37 degrees C. At pH 5.5 and 6.5 both immune whey product and HOSCN/OSCN- dose-dependently inhibited glucose uptake. The inhibition by their combination was additive if bacterial cells were treated with HOSCN/OSCN- before exposed to immune whey product. In contrast to immune whey product, the control product from sham-immunized cows increased the glucose uptake even when added simultaneously with HOSCN/OSCN-. However, when bacterial cells were pretreated with HOSCN/OSCN- an enhanced inhibitory effect was observed also with control product. The results indicate that colostral proteins from S. mutants- or S. sobrinus-immunized cows inhibit glucose uptake and that the effect is enhanced by pretreatment with lactoperoxidase-generated HOSCN/OSCN-.
免疫乳清产品是从用变形链球菌和远缘链球菌免疫的奶牛中获得的。在pH值为5.5或6.5的VMG缓冲液中，通过牛乳过氧化物酶、硫氰酸钾和过氧化氢生成次硫氰酸盐（HOSCN/OSCN-）。通过在37℃下测量14C-葡萄糖的摄取量，跟踪c血清型变形链球菌10449对数后期细胞的葡萄糖掺入情况。在pH值为5.5和6.5时，免疫乳清产品和HOSCN/OSCN-均呈剂量依赖性抑制葡萄糖摄取。如果在将细菌细胞暴露于免疫乳清产品之前先用HOSCN/OSCN-处理，则它们组合产生的抑制作用是相加的。与免疫乳清产品相反，来自假免疫奶牛的对照产品即使与HOSCN/OSCN-同时添加也会增加葡萄糖摄取。然而，当细菌细胞用HOSCN/OSCN-预处理时，对照产品也观察到增强的抑制作用。结果表明，来自用变形链球菌或远缘链球菌免疫的奶牛的初乳蛋白会抑制葡萄糖摄取，并且通过用过氧化物酶生成的HOSCN/OSCN-预处理可增强该作用。
