McMahon Amy, Supatto Willy, Fraser Scott E, Stathopoulos Angelike
Division of Biology, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Science. 2008 Dec 5;322(5907):1546-50. doi: 10.1126/science.1167094.
The concerted movement of cells from different germ layers contributes to morphogenesis during early embryonic development. Using an optimized imaging approach and quantitative methods, we analyzed the trajectories of hundreds of ectodermal cells and internalized mesodermal cells within Drosophila embryos over 2 hours during gastrulation. We found a high level of cellular organization, with mesoderm cell movements correlating with some but not all ectoderm movements. During migration, the mesoderm population underwent two ordered waves of cell division and synchronous cell intercalation, and cells at the leading edge stably maintained position. Fibroblast growth factor (FGF) signaling guides mesodermal cell migration; however, we found some directed dorsal migration in an FGF receptor mutant, which suggests that additional signals are involved. Thus, decomposing complex cellular movements can provide detailed insights into collective cell migration.
在胚胎发育早期，来自不同胚层的细胞协同运动有助于形态发生。我们采用优化的成像方法和定量方法，分析了果蝇胚胎原肠胚形成过程中数百个外胚层细胞和内化中胚层细胞在2小时内的运动轨迹。我们发现细胞具有高度的组织性，中胚层细胞的运动与部分而非全部外胚层细胞的运动相关。在迁移过程中，中胚层细胞群体经历了两轮有序的细胞分裂和同步的细胞插入，并且前沿的细胞稳定地保持其位置。成纤维细胞生长因子（FGF）信号传导引导中胚层细胞迁移；然而，我们在FGF受体突变体中发现了一些定向的背侧迁移，这表明还涉及其他信号。因此，分解复杂的细胞运动可以为集体细胞迁移提供详细的见解。
