Kodama Hiroshi, Okazaki Fumi, Ishida Saeko
Laboratory of Veterinary Immunology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
J Vet Med Sci. 2008 Nov;70(11):1185-90. doi: 10.1292/jvms.70.1185.
Humic substances are formed during the decomposition of organic matter in humus, and are found in many natural environments in which organic materials and microorganisms are present. Oral administration of humus extract to mice successfully induced effective protection against experimental challenge by the two subspecies, Trypanosoma brucei brucei and T. brucei gambiense. Mortality was most reduced among mice who received a 3% humus extract for 21 days in drinking water ad libitum. Spleen cells from humus-administered mice exhibited significant non-specific cytotoxic activity against L1210 mouse leukemia target cells. Also, spleen cells produced significantly higher amounts of Interferon-gamma when stimulated in vitro with Concanavalin A than cells from normal controls. These results clearly show that administration to mice of humus extract induced effective resistance against Trypanosoma infection. Enhancement of the innate immune system may be involved in host defense against trypanosomiasis.
腐殖质是在腐殖土中有机物分解过程中形成的，存在于许多有有机物质和微生物的自然环境中。给小鼠口服腐殖土提取物成功诱导了对布氏布氏锥虫和布氏冈比亚锥虫这两个亚种实验性攻击的有效保护。在自由饮用含3%腐殖土提取物的饮用水21天的小鼠中，死亡率降低最多。接受腐殖土处理的小鼠的脾细胞对L1210小鼠白血病靶细胞表现出显著的非特异性细胞毒性活性。此外，与正常对照的细胞相比，用伴刀豆球蛋白A体外刺激时，脾细胞产生的γ-干扰素量显著更高。这些结果清楚地表明，给小鼠施用腐殖土提取物可诱导对锥虫感染的有效抗性。先天免疫系统的增强可能参与宿主对锥虫病的防御。
