Steven F S, Bell J, Ellis I O
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
Anticancer Res. 1991 Sep-Oct;11(5):1697-9.
Cells obtained by fine needle aspiration of breast lumps were spread onto microscope slides, defatted with xylene and stained with a fluorescent probe for a cell surface protease. In these aspirates, carcinoma cells possess an active cell surface protease, guanidinobenzoatase (GB), very similar to plasminogen activator (1), which binds the fluorescent probe as a competitive inhibitor. Cells obtained from benign and normal breast lack this active GB and can be distinguished easily from the carcinoma cells by fluorescent microscopy. We use this simple technique to examine breast lumps containing carcinoma cells and to demonstrate the similarity of GB on these carcinoma cells to tissue type plasminogen activator (t-PA).
通过细针穿刺乳腺肿块获取的细胞被铺展在显微镜载玻片上，用二甲苯脱脂，并用一种针对细胞表面蛋白酶的荧光探针染色。在这些穿刺物中，癌细胞具有一种活性细胞表面蛋白酶——胍基苯甲酰酶（GB），它与纤溶酶原激活物非常相似（1），作为竞争性抑制剂与荧光探针结合。从良性和正常乳腺获取的细胞缺乏这种活性GB，通过荧光显微镜可以很容易地与癌细胞区分开来。我们使用这种简单的技术来检查含有癌细胞的乳腺肿块，并证明这些癌细胞上的GB与组织型纤溶酶原激活物（t-PA）的相似性。
