Nedashkovskaya Olga I, Kwon Kae Kyoung, Yang Sung-Hyun, Lee Hee-Soon, Chung Kyung Ho, Kim Sang-Jin
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan 425-600, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2694-8. doi: 10.1099/ijs.0.65799-0.
Two heterotrophic, aerobic, yellow-pigmented, Gram-negative, non-gliding bacteria, designated AKS293(T) and AKS432(T), isolated from a red alga, were analysed using a polyphasic taxonomic approach. 16S rRNA gene sequence analysis revealed that the novel strains were affiliated to the genus Lacinutrix, a member of the family Flavobacteriaceae, showing sequence similarities of 96.1-96.4 % with respect to the type strain of Lacinutrix copepodicola. The two novel isolates shared 99.5 % 16S rRNA gene sequence similarity and 55.0 % DNA-DNA relatedness. They grew optimally at 17.5 degrees C and pH 6.5. The main cellular fatty acids of strain AKS293(T) were iso-C(15 : 0), iso-C(15 : 0) 3-OH and iso-C(16 : 0) 3-OH, while those of strain AKS432(T) were anteiso-C(15 : 0), iso-C(15 : 0), iso-C(15 : 1) and iso-C(15 : 0) 3-OH. In both cases, the major isoprenoid quinone was MK-6. The DNA G+C contents were 34.7 and 37.0 mol% for strains AKS293(T) and AKS432(T), respectively. The phylogenetic evidence, phenotypic data and DNA-DNA hybridization results support the differentiation of strains AKS293(T) and AKS432(T) from each other and from their closest relative, L. copepodicola DJ3(T). Therefore, strains AKS293(T) and AKS432(T) represent two novel species, for which the names Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov. are proposed, respectively. The type strain of L. algicola sp. nov. is AKS293(T) (=KCCM 42313(T)=JCM 13825(T)) and the type strain of L. mariniflava sp. nov. is AKS432(T) (=KCCM 42306(T)=JCM 13824(T)). An emended description of the genus Lacinutrix is also proposed.
从红藻中分离出两株异养、需氧、黄色色素、革兰氏阴性、不滑动的细菌，分别命名为AKS293(T)和AKS432(T)，采用多相分类方法对其进行分析。16S rRNA基因序列分析表明，这两株新菌株隶属于黄杆菌科的拉克氏菌属，与桡足类拉克氏菌模式菌株的序列相似性为96.1 - 96.4%。这两株新分离株的16S rRNA基因序列相似性为99.5%，DNA - DNA相关性为55.0%。它们在17.5℃和pH 6.5条件下生长最佳。AKS293(T)菌株的主要细胞脂肪酸为异-C(15 : 0)、异-C(15 : 0) 3-OH和异-C(16 : 0) 3-OH，而AKS432(T)菌株的主要细胞脂肪酸为anteiso-C(15 : 0)、异-C(15 : 0)、异-C(15 : 1)和异-C(15 : 0) 3-OH。在这两种情况下，主要的类异戊二烯醌均为MK-6。AKS293(T)和AKS432(T)菌株的DNA G + C含量分别为34.7和37.0 mol%。系统发育证据、表型数据和DNA - DNA杂交结果支持AKS293(T)和AKS432(T)菌株彼此之间以及与它们最接近的亲缘种——桡足类拉克氏菌DJ3(T)的区分。因此，AKS293(T)和AKS432(T)菌株代表两个新物种，分别提议命名为藻栖拉克氏菌（Lacinutrix algicola sp. nov.）和海生黄拉克氏菌（Lacinutrix mariniflava sp.nov.）。藻栖拉克氏菌新种的模式菌株为AKS293(T) (=KCCM 42313(T)=JCM 13825(T))；海生黄拉克氏菌新种的模式菌株为AKS432(T) (=KCCM 42306(T)=JCM 13824(T))。同时还提议对拉克氏菌属进行修订描述。
