Chevrot Romain, Carlotti Arnaud, Sopena Valérie, Marchand Pierre, Rosenfeld Eric
Université de La Rochelle, Institut Littoral Environnement et Sociétés, LIENSS-UMR CNRS 6250, La Rochelle cedex 1, France.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2921-4. doi: 10.1099/ijs.0.2008/001297-0.
We report here the identification, characterization and culture of a Gram-negative to Gram-variable, rod-shaped, non-spore-forming anaerobic bacterium (strain FM1025(T)) isolated from the caecum of a duck. Phylogenetic analysis based on comparative 16S rRNA gene sequencing showed that this strain clustered with species of the family 'Acidaminococcaceae', with 94.9 % similarity to Megamonas hypermegale DSM 1672(T) and less than 91 % similarity with type strains of Pectinatus species. Sequence similarities of at least 98-99 % were observed with numerous sequences deposited in GenBank of uncultured strains from human and chicken caecal contents, but this strain is the first isolate of this taxon to be cultivated and described. On the basis of morphological, physiological and phylogenetic features, this strain should be assigned to a novel species in the genus Megamonas, for which the name Megamonas rupellensis sp. nov. is proposed. The type strain is strain FM1025(T) (=DSM 19944(T) =CIP 109788(T)).
我们在此报告从一只鸭的盲肠中分离出的一株革兰氏阴性至革兰氏可变、杆状、不形成芽孢的厌氧细菌（菌株FM1025(T)）的鉴定、特性分析及培养情况。基于比较16S rRNA基因测序的系统发育分析表明，该菌株与“氨基酸球菌科”的物种聚类，与巨大巨单胞菌DSM 1672(T)的相似性为94.9%，与果胶杆菌属模式菌株的相似性低于91%。在GenBank中来自人和鸡盲肠内容物的众多未培养菌株序列中观察到至少98 - 99%的序列相似性，但该菌株是该分类单元中首个被培养和描述的分离株。基于形态、生理和系统发育特征，该菌株应归入巨单胞菌属的一个新物种，为此提议将其命名为鲁佩尔巨单胞菌（Megamonas rupellensis sp. nov.）。模式菌株为菌株FM1025(T)（=DSM 19944(T) =CIP 109788(T)）。
