Depprich Rita, Ommerborn Michelle, Zipprich Holger, Naujoks Christian, Handschel Jörg, Wiesmann Hans-Peter, Kübler Norbert R, Meyer Ulrich
Department of Operative and Preventive Dentistry and Endodontics, Heinrich-Heine-University, Düsseldorf, Germany.
Head Face Med. 2008 Dec 8;4:29. doi: 10.1186/1746-160X-4-29.
Osseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problematic. The aim of the present study was to compare osteoblast behavior on structured zirconia and titanium surfaces under standardized conditions.
The surface characteristics were determined by scanning electron microscopy (SEM). In primary bovine osteoblasts attachment kinetics, proliferation rate and synthesis of bone-associated proteins were tested on different surfaces.
The results demonstrated that the proliferation rate of cells was significantly higher on zirconia surfaces than on titanium surfaces (p < 0.05; Student's t-test). In contrast, attachment and adhesion strength of the primary cells was significant higher on titanium surfaces (p < 0.05; U test). No significant differences were found in the synthesis of bone-specific proteins. Ultrastructural analysis revealed phenotypic features of osteoblast-like cells on both zirconia and titanium surfaces.
The study demonstrates distinct effects of the surface composition on osteoblasts in culture. Zirconia improves cell proliferation significantly during the first days of culture, but it does not improve attachment and adhesion strength. Both materials do not differ with respect to protein synthesis or ultrastructural appearance of osteoblasts. Zirconium oxide may therefore be a suitable material for dental implants.
骨结合对于牙种植体的长期成功至关重要，并且取决于组织-种植体界面处的组织反应。尽管表面处理仍存在问题，但机械性能和生物相容性使氧化锆成为牙种植体的合适材料。本研究的目的是在标准化条件下比较成骨细胞在结构化氧化锆和钛表面上的行为。
通过扫描电子显微镜（SEM）确定表面特征。在原代牛成骨细胞中，测试了不同表面上的附着动力学、增殖率和骨相关蛋白的合成。
结果表明，氧化锆表面上细胞的增殖率显著高于钛表面（p < 0.05；学生t检验）。相反，原代细胞在钛表面上的附着和黏附强度显著更高（p < 0.05；U检验）。在骨特异性蛋白的合成方面未发现显著差异。超微结构分析揭示了氧化锆和钛表面上成骨样细胞的表型特征。
该研究证明了表面组成对培养中的成骨细胞有明显影响。氧化锆在培养的最初几天显著改善细胞增殖，但未改善附着和黏附强度。两种材料在成骨细胞的蛋白质合成或超微结构外观方面没有差异。因此，氧化锆可能是牙种植体的合适材料。
