Ponader Sabine, Vairaktaris Eleftherios, Heinl Peter, Wilmowsky Cornelius V, Rottmair Andreas, Körner Carolin, Singer Robert F, Holst Stefan, Schlegel Karl A, Neukam Friedrich W, Nkenke Emeka
Department of Oral and Maxillofacial Surgery, University of Erlangen-Nuremberg, Erlangen, Germany.
J Biomed Mater Res A. 2008 Mar 15;84(4):1111-9. doi: 10.1002/jbm.a.31540.
The aim of the study was to assess the suitability of different Ti-6Al-4V surfaces produced by the electron beam melting (EBM) process as matrices for attachment, proliferation, and differentiation of human fetal osteoblasts (hFOB 1.19). Human osteoblasts were cultured in vitro on smooth and rough-textured Ti-6Al-4V alloy disks. By means of cell number and vitality and SEM micrographs cell attachment and proliferation were observed. The differentiation rate was examined by using quantitative real-time PCR analysis for the gene expression of alkaline phosphatase (ALP), type I collagen (Coll-I), bone sialoprotein (BSP) and osteocalcin (OC). After 3 days of incubation there was a significant higher vitality (p < 0.02) and proliferation (p < 0.02) of hFOB cells on smooth surfaces (R(a) = 0.077 microm) and compact surfaces with adherent partly molten titanium particles on the surface (R(a) </= 24.9 microm). On these samples cells spread over almost the whole surface. On porous surfaces with higher R(a) values, cell proliferation was reduced significantly. Quantitative real-time PCR analysis showed that the expression of osteogenic differentiation markers was not influenced by surface characteristics. Gene expression did not differ more than twofold for the different samples. Compact titanium samples with adherent partly molten titanium particles on the surface (R(a) </= 24.9 microm) fabricated by the EBM process turned out to be best suited for cell proliferation, while highly rough surfaces (R(a) >/= 56.9 microm) reduced proliferation of hFOB cells. Surface characteristics of titanium can easily be changed by EBM in order to further improve proliferation.
本研究的目的是评估通过电子束熔炼（EBM）工艺制备的不同Ti-6Al-4V表面作为人胎儿成骨细胞（hFOB 1.19）附着、增殖和分化基质的适用性。将人成骨细胞在光滑和纹理粗糙的Ti-6Al-4V合金圆盘上进行体外培养。通过细胞数量、活力以及扫描电子显微镜（SEM）显微照片观察细胞附着和增殖情况。通过定量实时PCR分析碱性磷酸酶（ALP）、I型胶原蛋白（Coll-I）、骨唾液蛋白（BSP）和骨钙素（OC）的基因表达来检测分化率。孵育3天后，hFOB细胞在光滑表面（R(a)=0.077微米）和表面带有附着的部分熔融钛颗粒的致密表面（R(a)≤24.9微米）上具有显著更高的活力（p<0.02）和增殖能力（p<0.02）。在这些样品上，细胞几乎覆盖了整个表面。在具有较高R(a)值的多孔表面上，细胞增殖显著降低。定量实时PCR分析表明，成骨分化标志物的表达不受表面特征影响。不同样品的基因表达差异不超过两倍。通过EBM工艺制备的表面带有附着的部分熔融钛颗粒的致密钛样品（R(a)≤24.9微米）被证明最适合细胞增殖，而高度粗糙的表面（R(a)≥56.9微米）会降低hFOB细胞的增殖。钛的表面特征可以通过EBM轻松改变以进一步改善增殖。
