丹酚酸 B 处理心肌缺血的代谢组学特征。
Metabolomic profiles of myocardial ischemia under treatment with salvianolic acid B.
机构信息
Department of Medicinal Chemistry of Nature Product, School of Pharmacy, Second Military Medical University, No, 325 Guohe Road, Shanghai 200433, China.
出版信息
Chin Med. 2012 Mar 13;7(1):6. doi: 10.1186/1749-8546-7-6.
BACKGROUND
Radix Salvia miltiorrhiza (Danshen) has been used as a principal herb in treating cardiovascular diseases in Chinese medicine. Salvianolic acid B (SA-B), a water-soluble active component of Danshen, was found to have anti-myocardial ischemia (anti-MI) effect. This study aims to investigate mechanisms of SA-B on MI.
METHODS
Five conventional Western medicines (isosorbide dinitrate, verapamil, propranolol, captopril and trimethazine) with different mechanisms for treating cardiovascular diseases were selected as positive references to compare with SA-B in changing of the metabolomic profiles in MI rats under treatment. Potential mechanisms of SA-B were further investigated in H9C2 cell line.
RESULTS
The metabolomic profiles between SA-B- and propranolol-treated MI rats were similar, since there was a big overlap between the two groups in the PLS-DA score plot. Finally, it was demonstrated that SA-B exhibited a protective effect on MI mainly by decreasing the concentration of cyclic adenosine monophosphate (cAMP) and Ca2+ and inhibiting protein kinase A (PKA).
CONCLUSION
SA-B and propanolol exhibited similar metabolomic profiles, indicating that the two drugs might have a similar mechanism.
背景
丹参作为中药治疗心血管疾病的主要草药，其水溶性活性成分丹酚酸 B（SA-B）具有抗心肌缺血（抗-MI）作用。本研究旨在探讨 SA-B 对 MI 的作用机制。
方法
选择五种常规西药（硝酸异山梨酯、维拉帕米、普萘洛尔、卡托普利和三甲嗪）作为阳性对照，与 SA-B 比较治疗 MI 大鼠的代谢组学特征。进一步在 H9C2 细胞系中研究 SA-B 的潜在作用机制。
结果
SA-B 和普萘洛尔治疗 MI 大鼠的代谢组学图谱相似，因为两组在 PLS-DA 得分图中有很大的重叠。最后，结果表明 SA-B 对 MI 的保护作用主要是通过降低环磷酸腺苷（cAMP）和 Ca2+的浓度以及抑制蛋白激酶 A（PKA）来实现的。
结论
SA-B 和普萘洛尔表现出相似的代谢组学图谱，表明这两种药物可能具有相似的作用机制。
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