Cao Zhaoxia, Zhang Jian, Zeng Julan, Sun Lixian, Xu Fen, Cao Zhong, Zhang Ling, Yang Daowu
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Materials & Thermochemistry Laboratory, Dalian 116023, Liaoning Province, China.
Talanta. 2009 Jan 15;77(3):943-7. doi: 10.1016/j.talanta.2008.06.043. Epub 2008 Jul 5.
In this work, novel mesoporous silica hollow spheres (MSHS) were chosen as an immobilization matrix, to construct a mediator-free third-generation HRP biosensor. UV-vis spectroscopy revealed that horseradish peroxidase (HRP) entrapped in MSHS could retain its native structure. FTIR spectroscopy and nitrogen adsorption-desorption isotherms indicated that HRP are intercalated into the mesopores. The direct electron transfer of HRP entrapped in MSHS was observed. A pair of stable and well-defined redox peaks of HRP with a formal potential of about -0.150 V (vs. Ag/AgCl) in 0.1M pH 7.0 phosphate-buffered solution (PBS) were obtained. The biosensor exhibited a fast amperometric response to H(2)O(2) with a linear range of 3.9 x 10(-6) to 1.4 x 10(-4)M (R=0.997, N=20). The detection limit was 1.2 x 10(-6)M based S/N=3.
在本工作中，选用新型介孔二氧化硅空心球（MSHS）作为固定化基质，构建无媒介体的第三代辣根过氧化物酶（HRP）生物传感器。紫外可见光谱表明，包裹于MSHS中的辣根过氧化物酶（HRP）能够保持其天然结构。傅里叶变换红外光谱和氮吸附-脱附等温线表明，HRP嵌入到介孔中。观察到包裹于MSHS中的HRP的直接电子转移。在0.1M pH 7.0磷酸盐缓冲溶液（PBS）中获得了一对稳定且明确的HRP氧化还原峰，其形式电位约为-0.150 V（相对于Ag/AgCl）。该生物传感器对H₂O₂呈现快速安培响应，线性范围为3.9×10⁻⁶至1.4×10⁻⁴M（R = 0.997，N = 20）。基于信噪比S/N = 3，检测限为1.2×10⁻⁶M。
