Key Laboratory for Advanced Materials, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China.
Department of Biotechnology, School of Sciences, Beijing Technology and Business University, Beijing 100048, China.
Sensors (Basel). 2019 Feb 3;19(3):640. doi: 10.3390/s19030640.
In this work, three different aqueous solutions containing imidazole-based ILs with different alkyl chain lengths ([Cmim]Br, n = 2, 6, 12) were adopted as the medium for the synthesis of ionic liquid-polypyrrole (IL-PPy) composites. Herein, the ILs undertook the roles of the pyrrole solvent, the media for emulsion polymerization of PPy and PPy dopants, respectively. The electrochemical performances of the three IL-PPy composites on a glassy carbon electrode (GCE) were investigated by electrochemical experiments, which indicated that [Cmim]Br-PPy (C-PPy) composites displayed better electrochemical performance due to their larger surface area and firmer immobilization on the GCE. Further, C-PPy/GCE were decorated with Au microparticles by electrodeposition that can not only increase the conductivity, but also immobilize sufficient biomolecules on the electrode. Then, the obtained C-PPy-Au/GCE with outstanding electrochemical performance was employed as a horseradish peroxidase (HRP) immobilization platform to fabricate a novel C-PPy-Au-HRP/GCE biosensor for H₂O₂ detection. The results showed that the prepared C-PPy-Au-HRP/GCE biosensor exhibited high sensitivity, fast response, and a wide detection range as well as low detection limit towards H₂O₂. This work not only provides an outstanding biomolecule immobilization matrix for the fabrication of highly sensitive biosensors, but also advances the understanding of the roles of ILs in improving the electrochemical performance of biosensors.
在这项工作中，采用了三种不同烷基链长的咪唑基离子液体([Cmim]Br，n = 2,6,12)的水溶液作为合成离子液体-聚吡咯(IL-PPy)复合材料的介质。在这里，离子液体分别作为吡咯的溶剂、PPy 的乳液聚合介质和 PPy 掺杂剂。通过电化学实验研究了三种 IL-PPy 复合材料在玻碳电极(GCE)上的电化学性能，结果表明[Cmim]Br-PPy(C-PPy)复合材料由于其较大的比表面积和更牢固地固定在 GCE 上，表现出更好的电化学性能。此外，通过电沉积在 C-PPy/GCE 上修饰了金纳米粒子，这不仅可以增加导电性，还可以在电极上固定足够的生物分子。然后，将具有优异电化学性能的所得 C-PPy-Au/GCE 用作辣根过氧化物酶(HRP)固定化平台，以制备用于 H₂O₂检测的新型 C-PPy-Au-HRP/GCE 生物传感器。结果表明，所制备的 C-PPy-Au-HRP/GCE 生物传感器对 H₂O₂具有高灵敏度、快速响应、宽检测范围和低检测限。这项工作不仅为制备高灵敏度的生物传感器提供了出色的生物分子固定基质，还推进了对离子液体在改善生物传感器电化学性能中的作用的理解。
