Department of Otorhinolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, #227 Yanta West Road, Xi'an, 710061, Shaanxi, People's Republic of China.
Department of Neurosurgery, The Second Affiliated Hospital of Xi'an Jiaotong University, #157 Xiwu Road, Xi'an, 710004, Shaanxi, People's Republic of China.
Int J Clin Oncol. 2020 Apr;25(4):660-669. doi: 10.1007/s10147-019-01586-9. Epub 2019 Dec 12.
Head and neck squamous cell carcinomas (HNSCCs) are one of the most common cancers in the world, and nucleotide excision repair (NER) is involved in HNSCCs susceptibility. We investigated whether mRNA expression levels of nine core NER genes were associated with risk of HNSCCs in a Chinese population.
In this study of 251 HNSCC patients and 232 healthy controls, we quantified NER gene mRNA expression levels in cultured peripheral lymphocytes using a quantitative real-time PCR.
Compared with the controls, HNSCC patients had statistically significantly lower expression levels of XPA and XPB (P = 0.029 and 0.001, respectively). After dividing the subjects by the controls' median values of expression levels, we found a dose-dependent association between an increased risk of HNSCCs and low expression levels of XPB (adjusted OR 1.56 and 95% CI 1.07-2.28; P = 0.001). We also identified a significant multiplicative interaction between smoking status as well as alcohol status and mRNA expression levels of XPB (P = 0.014 and 0.042, respectively). Finally, after integrating demographic variables, we found the addition of smoking status and XPB expression levels to the model significantly improved the sensitivity of the expanded model on HNSCC risk.
Reduced mRNA expression levels of XPB were associated with an increased risk of HNSCCs in a Chinese population.
头颈部鳞状细胞癌（HNSCC）是世界上最常见的癌症之一，核苷酸切除修复（NER）参与了 HNSCC 的易感性。我们研究了在中国人群中，九个核心 NER 基因的 mRNA 表达水平是否与 HNSCC 的发病风险相关。
在这项对 251 例 HNSCC 患者和 232 例健康对照者的研究中，我们使用定量实时 PCR 检测培养的外周血淋巴细胞中 NER 基因的 mRNA 表达水平。
与对照组相比，HNSCC 患者的 XPA 和 XPB 表达水平显著降低（P=0.029 和 0.001）。根据对照组的表达水平中位数将研究对象进行分组后，我们发现 XPB 表达水平降低与 HNSCC 发病风险呈剂量依赖性相关（调整后的 OR 为 1.56，95%CI 为 1.07-2.28；P=0.001）。我们还发现吸烟状态和饮酒状态与 XPB mRNA 表达水平之间存在显著的相乘交互作用（P=0.014 和 0.042）。最后，在整合了人口统计学变量后，我们发现将吸烟状态和 XPB 表达水平加入模型后，显著提高了扩展模型对 HNSCC 风险的敏感性。
在中国人群中，XPB 的 mRNA 表达水平降低与 HNSCC 的发病风险增加相关。
