Laemle L K, Repke K B, Hawkes R, Rice F L
Department of Anatomy, UMDNJ-New Jersey Medical School, Newark 07103.
Brain Res. 1991 Mar 22;544(1):108-17. doi: 10.1016/0006-8993(91)90891-x.
MabQ155, a monoclonal antibody against synaptophysin, has been used to conduct a light microscopic survey of synaptogenesis in the suprachiasmatic nucleus of the perinatal rat. Synaptophysin is an integral component of synaptic vesicle membranes which is expressed in growth cones and growth cone filopodia as well as in mature synapses. With the light microscope, mabQ155 immunoreactivity in growth cones can be distinguished from that in presynaptic terminals on the basis of the size of immunoreactive puncta. The current study presents a qualitative and quantitative analysis of synaptogenesis from the day of birth (P0) to postnatal day 10 (P10). In our quantitative analysis we have used daily intervals during the first postnatal week, distinguished between growth cones and presynaptic terminals, and divided the suprachiasmatic nucleus into sampling regions that are related to the progress of synaptogenesis. Our data demonstrate regional differences in synaptogenesis within the suprachiasmatic nucleus (SCN), document the temporal progression from the penetration of growth cones to the appearance of mature synapses, and provide information about gradients of synaptogenesis in the nucleus during development.
MabQ155是一种抗突触素的单克隆抗体，已被用于对围产期大鼠视交叉上核的突触形成进行光学显微镜观察。突触素是突触小泡膜的一个组成成分，在生长锥、生长锥丝状伪足以及成熟突触中均有表达。利用光学显微镜，基于免疫反应性斑点的大小，生长锥中的mabQ155免疫反应性可与突触前终末中的免疫反应性区分开来。本研究对出生日（P0）至出生后第10天（P10）的突触形成进行了定性和定量分析。在我们的定量分析中，我们在出生后的第一周采用了每日间隔，区分了生长锥和突触前终末，并将视交叉上核划分为与突触形成进程相关的采样区域。我们的数据证明了视交叉上核（SCN）内突触形成的区域差异，记录了从生长锥侵入到成熟突触出现的时间进程，并提供了发育过程中该核内突触形成梯度的信息。
