Hedrick D B, Guckert J B, White D C
Institute for Applied Microbiology, University of Tennessee, Knoxville 37932-2567.
J Lipid Res. 1991 Apr;32(4):659-66.
A strategy has been developed for archaebacterial lipid analysis which provides three times the information to describe archaebacterial isolates and is compatible with simultaneous eubacterial/eukaryotic lipid analysis of environmental samples. Eubacterial and micro-eukaryotic biomass, community structure, and nutritional status have been routinely defined in environmental samples by lipid analysis. Lipid profiles are also useful in eubacterial identification and taxonomy. Polar lipid or whole cell ester-linked fatty acids are generally analyzed by gas chromatography-mass spectroscopy. Archaebacteria are characterized by their ether-linked membrane lipids. There is, however, less diversity in the side chains of archaebacterial membrane lipids as compared the eubacterial ester-linked membrane lipids. The information content of the archaebacterial lipid profile was increased by separately analyzing the polar lipid, glycolipid, and lipid-extracted residue fractions. Identification and quantification were performed by supercritical fluid chromatography. Results are presented for three species of methanogens and four thermoacidophile isolates, and compared with a literature review.
已开发出一种用于古细菌脂质分析的策略，该策略能提供三倍的信息来描述古细菌分离株，并且与环境样品中真细菌/真核生物脂质的同步分析兼容。通过脂质分析，已常规确定环境样品中的真细菌和微真核生物生物量、群落结构以及营养状况。脂质谱在真细菌的鉴定和分类学中也很有用。极性脂质或全细胞酯连接脂肪酸通常通过气相色谱 - 质谱法进行分析。古细菌的特征在于其醚连接的膜脂质。然而，与真细菌酯连接的膜脂质相比，古细菌膜脂质侧链的多样性较少。通过分别分析极性脂质、糖脂和脂质提取残余物部分，增加了古细菌脂质谱的信息含量。通过超临界流体色谱进行鉴定和定量。给出了三种产甲烷菌和四种嗜热嗜酸菌分离株的结果，并与文献综述进行了比较。
