Salimpour Fahimeh, Mostafavi Gelaleh, Sharifnia Fariba
Department of Biology, Faculty of Basic Sciences, Islamic Azad University, North Tehran Branch, P.O. Box 19585-936, Tehran, Iran.
Pak J Biol Sci. 2007 Feb 1;10(3):378-82. doi: 10.3923/pjbs.2007.378.382.
The characteristics of the seeds of 7 annual and perennial species of Trifolium, Lotoidea section, in Iran were investigated by means of Scanning Electron Microscopy (SEM) and a stereomicroscope. Contrary to Zohary's belief who maintained that the micromorphology of the Trifolium's seed played no role in taxonomy, the results revealed that such characteristics can play a determining role in differentiating such species and distinguishing them from one another. The results of the micromorphologic study of the seed confirmed the morphological results of the species of the Lotoidea section to a great extent, but was involved in the separation and differentiation of the two species of T. repens and T. nigrescens. In general, the characteristics of the species' seeds proved quite effective in determining their phenetic relationship. Among the characteristics studied in the present research, mention can be made of seed shape, hilum shape, seed sculpture and seed length and width (L/W ratio). The results demonstrated that the characteristics of seed length and hilum shape showed the greatest diversity in the species. The longest seed length was that of the species T. radicosum and the shortest that of T. repens. The spindle shape of the hilum was an exclusive characteristic of T. radicosum which distinguished it more than any other characteristic from all the other species of this section. The difference in hilum shape in the two species of T. repens and T. nigrescens was an important distinctive characteristic.
利用扫描电子显微镜（SEM）和体视显微镜，对伊朗车轴草属（Trifolium）百脉根组（Lotoidea section）7种一年生和多年生植物种子的特征进行了研究。与Zohary认为车轴草种子的微观形态在分类学中不起作用的观点相反，结果表明，这些特征在区分此类物种以及将它们彼此区分开来方面可以发挥决定性作用。种子微观形态学研究结果在很大程度上证实了百脉根组物种的形态学研究结果，但涉及到白车轴草（T. repens）和变黑车轴草（T. nigrescens）这两个物种的分离和区分。总体而言，物种种子的特征在确定它们的表型关系方面被证明相当有效。在本研究中所研究的特征中，可以提及种子形状、种脐形状、种子纹饰以及种子长度和宽度（长宽比）。结果表明，种子长度和种脐形状的特征在物种中表现出最大的多样性。种子长度最长的是根车轴草（T. radicosum），最短的是白车轴草。种脐的纺锤形是根车轴草独有的特征，这使其与本组的所有其他物种相比，在区分上比任何其他特征都更具辨识度。白车轴草和变黑车轴草这两个物种在种脐形状上的差异是一个重要的区别特征。
