Department of Applied Bioscience, Faculty ofAgriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
J Environ Sci (China). 2010;22(1):142-9. doi: 10.1016/s1001-0742(09)60085-1.
Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate (92%) was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9,10-phenanthrenequinone, 2,2'-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase (187.4 U/L) after 20 days of culture.
从污染土壤中采集的真菌 Polyporus sp. S133 被用于在无机盐肉汤液体培养基中降解多环芳烃菲。与非搅拌培养物中 44%的降解相比，当 Polyporus sp. S133 在 120 r/min 搅拌下培养 30 天时，获得了最大降解率（92%）。此外，添加表面活性剂会影响降解。吐温 80 是最适合 Polyporus sp. S133 降解菲的表面活性剂。随着添加的吐温 80 量的增加，降解率增加。搅拌培养物中的速率约是非搅拌培养物中的 2 倍。通过鉴定代谢产物确定了降解机制；9,10-菲醌、2,2'-联苯二酸、邻苯二甲酸和原儿茶酸。在孵育过程中检测到由 Polyporus sp. S133 产生的几种酶（锰过氧化物酶、木质素过氧化物酶、漆酶、1,2-加氧酶和 2,3-加氧酶）。在培养 20 天后，1,2-加氧酶（187.4 U/L）表现出最高的活性。
