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结合蛋白质组学和代谢物分析以揭示杨树叶片中的镉胁迫响应。

Combining proteomics and metabolite analyses to unravel cadmium stress-response in poplar leaves.

作者信息

Kieffer Pol, Planchon Sébastien, Oufir Mouhssin, Ziebel Johanna, Dommes Jacques, Hoffmann Lucien, Hausman Jean-François, Renaut Jenny

机构信息

Centre de Recherche Public-Gabriel Lippmann, L-4422 Belvaux, Luxembourg, Belgium.

出版信息

J Proteome Res. 2009 Jan;8(1):400-17. doi: 10.1021/pr800561r.

DOI:10.1021/pr800561r
PMID:19072159
Abstract

A proteomic analysis of poplar leaves exposed to cadmium, combined with biochemical analysis of pigments and carbohydrates revealed changes in primary carbon metabolism. Proteomic results suggested that photosynthesis was slightly affected. Together with a growth inhibition, photoassimilates were less needed for developmental processes and could be stored in the form of hexoses or complex sugars, acting also as osmoprotectants. Simultaneously, mitochondrial respiration was upregulated, providing energy needs of cadmium-exposed plants.

摘要

对暴露于镉的杨树叶片进行蛋白质组学分析,并结合对色素和碳水化合物的生化分析,揭示了初级碳代谢的变化。蛋白质组学结果表明光合作用受到轻微影响。伴随着生长抑制,发育过程对光合产物的需求减少,光合产物可以以己糖或复合糖的形式储存,同时也起到渗透保护剂的作用。同时,线粒体呼吸上调,满足暴露于镉的植物的能量需求。

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