Yuan Jinbin, Nie Lihua, Zeng Deyu, Luo Xubiao, Tang Fei, Ding Li, Liu Qian, Guo Manli, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing & Chemometrics, Chemistry & Chemical Engineering College, Hunan University, Changsha 410082, PR China; Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, PR China.
Talanta. 2007 Oct 15;73(4):644-50. doi: 10.1016/j.talanta.2007.04.042. Epub 2007 May 5.
By optimizing the extraction, separation and analytical conditions, a simple, reliable and effective high-performance liquid chromatography method coupled with photodiode array detector (HPLC-DAD) is presented for simultaneous determination of nine aristolochic acid (AA) analogues, i.e., AA I, AA II, AA C, AA D, 7-OH AA I, aristolic acid, AL II, AL III and AL IV, in twelve medicinal herbs and two preparations. The separation was completed on a C18 column with aqueous methanol containing 0.2% (V/V) acetic acid as mobile phase. Linearities of around two orders of magnitude were obtained with correlation coefficients exceeding 0.9950. Satisfactory intra-day and inter-day precisions were achieved with R.S.D.s less than 4.35%, and the average recovery factors obtained were in the range of 88.4-98.8%. The proposed method appears to be suitable for use as a tool for safety assurance and quality control for commercially available suspect samples containing aristolochic acid analogues.
通过优化提取、分离和分析条件，建立了一种简单、可靠且有效的高效液相色谱法结合光电二极管阵列检测器（HPLC-DAD），用于同时测定12种草药和2种制剂中的9种马兜铃酸（AA）类似物，即AA I、AA II、AA C、AA D、7-羟基AA I、马兜铃酸、AL II、AL III和AL IV。分离在C18柱上进行，以含0.2%（V/V）乙酸的甲醇水溶液为流动相。获得了约两个数量级的线性关系，相关系数超过0.9950。日内和日间精密度良好，相对标准偏差（R.S.D.）小于4.35%，平均回收率在88.4%至98.8%之间。该方法似乎适用于作为含有马兜铃酸类似物的市售可疑样品的安全保证和质量控制工具。
